A-484954
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 100mg | $1000 | Check With Us |
| 250mg | $1850 | Check With Us |
| 500mg | $2775 | Check With Us |
: Cat #: V32904 CAS #: 142557-61-7 Purity ≥ 98%
Description: A-484954 (A484954; A 484954) is a highly selective, cell-permeable and ATP-competitive eukaryotic elongation factor-2 (eEF2) inhibitor with potential antitumor activity.
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| Molecular Weight (MW) | 289.29 |
|---|---|
| Molecular Formula | C₁₃H₁₅N₅O₃ |
| CAS No. | 142557-61-7 |
| SMILES Code | O=C(N1CC)N(C2CC2)C3=C(C=C(C(N)=O)C(N)=N3)C1=O |
| Protocol | In Vitro | A-484954 is a highly selective eEF2K inhibitor with an IC50 value of 280 nM against eEF2K in the enzymatic assay and little activity against a wide panel of serine/threonine and tyrosine kinases. In enzymatic assay, the IC50 value of A-484954 is increased as the concentration of ATP increased but unaffected by increasing concentrations of calmodulin |
|---|---|---|
| In Vivo | A484954 causes relaxation in E (+) and E (-) aorta or mesenteric artery precontracted with NA. Pretreatment with L-NAME but not indomethacin or cimetidine partially inhibits the A484954-induced relaxation in mesenteric artery |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.4567 mL | 17.2837 mL | 34.5674 mL | 69.1348 mL |
| 5mM | 0.6913 mL | 3.4567 mL | 6.9135 mL | 13.8270 mL |
| 10mM | 0.3457 mL | 1.7284 mL | 3.4567 mL | 6.9135 mL |
| 20mM | 0.1728 mL | 0.8642 mL | 1.7284 mL | 3.4567 mL |
The molarity calculator equation
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The dilution calculator equation
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.