Description: NSC77674 (also known as PIH or Pyridoxal isonicotinoyl hydrazone) is is a novel, potent, lipophilic, nontoxic, iron-chelating agent that shows high iron chelation efficacy both in vitro in cell culture models and in vivo in rats and mice. As iron is a crucial component of metabolic pathways involved in DNA synthesis, cancerous cells have a high iron requirement due to rapid rates of proliferation. While pyridoxal isonicotinoyl hydrazine exhibits low anticancer activity, its analogs demonstrate antiproliferative effects in a range of tumor cells. As a cell-permeable, non-toxic tridendate iron (Fe3+) chelator of the aroyl hydrazone class, even at low concentrations, NSC77674 is highly effective in mobilizing Fe3+ from cells and preventing its uptake from transferrin. It exhibits similar specificity and binding affinity as Deferoxamine Mesylate but has greater access to mitochondrial iron. It is also useful for the management of iron overload disease in experimental models.