This product is for research use only, not for human use. We do not sell to patients.
| Size | Price | Stock |
|---|---|---|
| 5mg | $98 | 3-6 Days |
| 10mg | $140 | 3-6 Days |
| 25mg | $280 | 3-6 Days |
| 50mg | $420 | 3-6 Days |
| 100mg | $738 | 3-6 Days |
| 250mg | $1290 | 3-6 Days |
| 500mg | $1850 | 3-6 Days |
: Cat #: V2670 CAS #: 5428-80-8 (free base) Purity ≥ 98%
Description: Vacquinol-1 dihydrochloride (also known as NSC-13316; NSC13316) is an MKK4 (mitogen-activated protein kinase kinase 4) activator with antitumor activity. It rapidly and selectively induces glioma cell death. Also inducing apoptosis in hepatocellular carcinoma (HCC)cell. Research showed that vacquinol-1 displayed high cytotoxicity against glioma cells, resulting in a complete loss of viability as measured by ATP depletion. Vacquinol-1 could selectively target GCs in mixed cocultures with human fibroblasts. Moreover, vacquinol-1 had no effect on caspase activity at any concentration or time point, which was unlike that of staurosporin. In addition, the fluorescence staining and western blot analyses of GCs for activating MKK4 phosphorylation revealed a rapid and pronounced activation by vacquinol-1 at 7.5 μM.
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| Molecular Weight (MW) | 425.78 |
|---|---|
| Molecular Formula | C21H21ClN2O · 2HCl |
| CAS No. | 5428-80-8 (free base) |
| Storage | -20℃ for 3 years in powder form |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 70 mg/mL (164.4 mM) |
| Water: <1 mg/mL | |
| Ethanol: <1 mg/mL | |
| Solubility In Vivo | ClC1=CC=C(C2=NC(C=CC=C3)=C3C(C(O)C4NCCCC4)=C2)C=C1.[H]Cl |
| Synonyms | NSC13316 hydrochloride; NSC 13316; NSC-13316; Vacquinol-1; Vacquinol 1; Vacquinol1 hydrochloride |
| Protocol | In Vitro | In vitro activity: Vacquinol-1 induces rapid and specific cell death of glioma cells with IC50 of 3.14 μM via membrane ruffling, cell rounding, massive macropinocytic vacuole accumulation, ATP depletion, and cytoplasmic membrane rupture. Kinase Assay: Vacquinol-1 (also known as NSC-13316; NSC13316) is an MKK4 (mitogen-activated protein kinase kinase 4) activator with antitumor activity. Cell Assay: Cell viability is measured using CellTiter-Glo Luminescent Cell Viability Assay according to the manufacturer’s instructions. |
|---|---|---|
| In Vivo | The ability of vacquinol-1 to attenuate tumor progression was previously tested in a mouse model for human GBM. Vacquinol-1 or vehicle (DMSO) were intracranially administered into the site of original cell deposit 6 weeks after engraftment. Results showed that tumors were invariantly smaller, and the area of necrosis and hGFAP and hNestin immunoreactivity was significantly reduced. Moreove, only tumor cells in vacquinol-1-treated mice showed a massive LAMP1 staining. | |
| Animal model | Mice bearing U3013MG tumors |
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.3486 mL | 11.7432 mL | 23.4863 mL | 46.9726 mL |
| 5mM | 0.4697 mL | 2.3486 mL | 4.6973 mL | 9.3945 mL |
| 10mM | 0.2349 mL | 1.1743 mL | 2.3486 mL | 4.6973 mL |
| 20mM | 0.1174 mL | 0.5872 mL | 1.1743 mL | 2.3486 mL |
This equation is commonly abbreviated as: C1 V1 = C2 V2
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.