Bisbenzimide HCl (Pibenzimol, Hoechst 33258)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
50mg	$50	3-6 Days
100mg	$75	3-6 Days
250mg	$120	3-6 Days
500mg	$200	3-6 Days
1g	$350	3-6 Days

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Cat #: V8097 CAS #: 23491-45-4 Purity ≥ 98%

Description: Bisbenzimide, formerly known as Pibenzimol, Hoechst 33258, is a cell-permeable, benzimidazole dye that binds to the minor groove of double stranded DNA with preference for adenine and thymine-rich sequences.

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Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	533.882
Molecular Formula	C25H27Cl3N6O
CAS No.	23491-45-4

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.8731 mL	9.3654 mL	18.7307 mL	37.4615 mL
5mM	0.3746 mL	1.8731 mL	3.7461 mL	7.4923 mL
10mM	0.1873 mL	0.9365 mL	1.8731 mL	3.7461 mL
20mM	0.0937 mL	0.4683 mL	0.9365 mL	1.8731 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.