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| Size | Price | Stock |
|---|---|---|
| 5g | $140 | 3-6 Days |
| 10g | $250 | 3-6 Days |
| 25g | $450 | 3-6 Days |
| 50g | $750 | 3-6 Days |
: Cat #: V29509 CAS #: 7240-90-6 Purity ≥ 99%
Description: X-GAL (5-bromo-4-chloro-3-indolyl-β-D-galactoside), consisting of galactose linked to a substituted indole, is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays., and thus is widely used a chromogenic β-galactosidase substrate. β-galactosidase cleaves X-gal and produce an insoluble blue compound, which is detectable. X-gal is often used in molecular biology to test for the presence of an enzyme, β-galactosidase. It is also used to detect activity of this enzyme in histochemistry and bacteriology. X-gal is one of many indoxyl glycosides and esters that yield insoluble blue compounds similar to indigo dye as a result of enzyme-catalyzed hydrolysis.
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| Molecular Weight (MW) | 408.63 |
|---|---|
| Molecular Formula | C₁₄H₁₅BrClNO₆ |
| CAS No. | 7240-90-6 |
| Synonyms | BCIG |
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.4472 mL | 12.2360 mL | 24.4720 mL | 48.9440 mL |
| 5mM | 0.4894 mL | 2.4472 mL | 4.8944 mL | 9.7888 mL |
| 10mM | 0.2447 mL | 1.2236 mL | 2.4472 mL | 4.8944 mL |
| 20mM | 0.1224 mL | 0.6118 mL | 1.2236 mL | 2.4472 mL |
This equation is commonly abbreviated as: C1 V1 = C2 V2
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.