This product is for research use only, not for human use. We do not sell to patients.
| Size | Price | Stock |
|---|---|---|
| 10mg | $32 | 3-6 Days |
| 25mg | $50 | 3-6 Days |
| 50mg | $80 | 3-6 Days |
| 100mg | $120 | 3-6 Days |
| 250mg | $180 | 3-6 Days |
| 500mg | $280 | 3-6 Days |
| 1g | $450 | 3-6 Days |
: Cat #: V1866 CAS #: 2591-17-5 Purity ≥ 98%
Description: D-Luciferin is a popular and cell-permeable bioluminescent substrate of luciferase in the presence of ATP with a Km of approximately 2 μM, it is used in luciferase-based bioluminescence imaging and cell-based high-throughput screening applications. D-luciferin could emit lights upon oxidative decarboxylation in the presence of ATP. D-luciferin provides a bioluminescent signal for in vivo and in vitro detection of cellular ATP levels. D-Luciferin chould be used to assay the expression of the luciferase gene linked to a promoter of interest. Alternatively, D-luciferin and luciferase can be used to assess ATP availability in cellular or biochemical assays. D-luciferin could be administrated intravenously or intraperitonealy.
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- Physicochemical and Storage Information
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- Related Biological Data
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- Quality Control Documentation
| Molecular Weight (MW) | 280.32 |
|---|---|
| Molecular Formula | C11H8N2O3S2 |
| CAS No. | 2591-17-5 |
| Storage | -20℃ for 3 years in powder form |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 56 mg/mL (199.8 mM) |
| Water: <1 mg/mL | |
| Ethanol: <1 mg/mL | |
| Synonyms | D-(-)-Luciferin; Firefly luciferin |
| Protocol | In Vitro | In vitro activity: D-luciferin reacts with luciferase, ATP and oxygen with light emission, and the light is detected by a sensitive photographic film, thereby permitting the visualization of the alkaline phosphatase-conjugated antibodies. |
|---|---|---|
| In Vivo | In an immunocompetent mouse model of ovarian cancer, use of D-luciferin substrate and firefly luciferase preserves tumor-host immune interactions, since bioluminescent imaging is a more sensitive indication of tumor growth than weight gain. | |
| Animal model | Mice |
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.5674 mL | 17.8368 mL | 35.6735 mL | 71.3470 mL |
| 5mM | 0.7135 mL | 3.5674 mL | 7.1347 mL | 14.2694 mL |
| 10mM | 0.3567 mL | 1.7837 mL | 3.5674 mL | 7.1347 mL |
| 20mM | 0.1784 mL | 0.8918 mL | 1.7837 mL | 3.5674 mL |
This equation is commonly abbreviated as: C1 V1 = C2 V2
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.