Tenofovir Disoproxil Fumarate (Tenofovir DF)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
250mg	$200	Check With Us
500mg	$345	Check With Us
1g	$515	Check With Us

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Cat #: V1812 CAS #: 202138-50-9 Purity ≥ 98%

Description: Tenofovir Disoproxil Fumarate (TDF; GS-433105; Viread; Tenofovir DF), the Fumarate salt of tenofovir, belongs to a class of antiretroviral drugs, it inhibits the activity of HIV reverse transcriptase by competing with the natural substrate deoxyadenosine 5'-triphosphate and, after incorporation into DNA, by DNA chain termination.

References: Murphy RA, et al. Establishment of HK-2 Cells as a Relevant Model to Study Tenofovir-Induced Cytotoxicity. Int J Mol Sci. 2017 Mar 1;18(3)

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	635.51
Molecular Formula	C19H30N5O10P.C4H4O4
CAS No.	202138-50-9
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 128 mg/mL (201.4 mM)r
	Water: 10-20 mg/mL (with ultrasonic and warming)r
	Ethanol: 44 mg/mL (69.2 mM)
Synonyms	GS4331-05; GS-1278 Disoproxil Fumarate; Tenofovir DF; TDF; GS-4331-05; GS 4331-05; Tenofovir Disoproxil Fumarate; PMPA prodrug; Tenofovir DF; GS-433105; Viread; Bis(POC)-PMPA

Protocol	In Vitro	Tenofovir shows cytotoxic effects on cell viability in HK-2 cells, with IC50 values of 9.21 and 2.77 μM at 48 and 72 h in MTT assay, respectively. Tenofovir diminishes ATP levels in HK-2 cells. Tenofovir (3.0 to 28.8 μM) increases oxidative stress and protein carbonylation in HK-2 cells. Furthermore, Tenofovir induces apoptosis in HK-2 cells, and that apoptosis is induced via mitochondrial damage

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.5735 mL	7.8677 mL	15.7354 mL	31.4708 mL
5mM	0.3147 mL	1.5735 mL	3.1471 mL	6.2942 mL
10mM	0.1574 mL	0.7868 mL	1.5735 mL	3.1471 mL
20mM	0.0787 mL	0.3934 mL	0.7868 mL	1.5735 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.