Ouabain (G-Strophanthin)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
5g	$645	Check With Us
10g	$945	Check With Us
20g	$1415	Check With Us

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Cat #: V6287 CAS #: 630-60-4 Purity ≥ 98%

Description: Ouabain (Ouabain Octahydrate; Acocantherine; G-Strophanthin), a cardiac steroid, is a novel, potent and selective Na+/K+, -ATPase inhibitor, which binds to α2 /α3 subunit with Ki of 41 nM/15 nM.

References: Juhaszova M, et al. Proc Natl Acad Sci U S A, 1997, 94

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	584.66
Molecular Formula	C29H44O12
CAS No.	630-60-4
Synonyms	Ouabain; Acocantherin; Strodival; NSC 25485; NSC-25485; NSC25485

Protocol	In Vitro	In cultured rat astrocyte, Ouabain (0.1 μM-1.0 μM) inhibits Na+ pumping and increases storedCa2+. The high-wabaine affinity subtypes (α2 in astrocytes and α3 in neurons and myocytes) are confined to a reticular distribution within PM, parallel to the underlying endoplasmic reticulum or sarcoplasmic reticulum.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.7104 mL	8.5520 mL	17.1040 mL	34.2079 mL
5mM	0.3421 mL	1.7104 mL	3.4208 mL	6.8416 mL
10mM	0.1710 mL	0.8552 mL	1.7104 mL	3.4208 mL
20mM	0.0855 mL	0.4276 mL	0.8552 mL	1.7104 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.