BTB06584 | CAS 219793-45-0

BTB06584

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
250mg	$400	Check With Us
500mg	$650	Check With Us
1g	$975	Check With Us

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Cat #: V0191 CAS #: 219793-45-0 Purity ≥ 98%

Description: BTB06584 (BTB-06584; BTB 06584) is a novel, potent and IF1-dependent inhibitor of the mitochondrial F1 Fo-ATPase, acting by selectively inhibiting mitochondrial F1 Fo-ATPase activity without compromising ATP synthesis and to limit ischaemia-induced injury caused by reversal of the mitochondrial F1 Fo-ATPsynthase.

References: Ivanes F, et al. The compound BTB06584 is an IF1 -dependent selective inhibitor of the mitochondrial F1 Fo-ATPase. Br J Pharmacol. 2014 Sep;171(18):4193-4206.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	417.82
Molecular Formula	C19H12ClNO6S
CAS No.	219793-45-0
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 84 mg/mL (201.0 mM)r
	Water: <1 mg/mLr
	Ethanol: <1 mg/mL
Synonyms	BTB06584; BTB 06584; BTB-06584

Protocol	In Vitro	In HL-1 cells, BTB06584 (100 μM) inhibits F1Fo-ATPase activity with no effect on the mitochondrial membrane potential (ΔΨm ) or O2 consumption. BTB06584 (100 μM) pretreatmet protects against ischaemic cell death in HL-1 cells prior to a period of ischaemia. ATP consumption is decreased following inhibition of respiration, and ischaemic cell death is reduced
Protocol	In Vivo	BTB06584 (1 μM; 24 hours) treatment rescued defective haemoglobin synthesis in zebrafish pinotage (pnt) mutants in which expression of the Atpif1a gene is lost. The concentrations of BTB06584 that restore haemoglobin biosynthesis also alter mitochondrial bioenergetics in living fish

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.3934 mL	11.9669 mL	23.9338 mL	47.8675 mL
5mM	0.4787 mL	2.3934 mL	4.7868 mL	9.5735 mL
10mM	0.2393 mL	1.1967 mL	2.3934 mL	4.7868 mL
20mM	0.1197 mL	0.5983 mL	1.1967 mL	2.3934 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.