NNC 55-0396 | CAS 357400-13-6

NNC 55-0396

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
250mg	$1250	Check With Us
500mg	$1950	Check With Us
1g	$2975	Check With Us

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Cat #: V3671 CAS #: 357400-13-6 Purity ≥ 98%

Description: NNC 55-0396 dihydrochloride salt, an analog of mibefradil, is a novel, potent and highly selective T-type calcium channel blocker which displays IC50 values of 6.8 and > 100 μM for inhibition of Cav3.1 T-type channels and HVA currents respectively in INS-1 cells.

References: Arnulfo Quesadaa, Peter H. Bui, Gregg E. Homanics，et al. Comparison of mibefradil and derivative NNC 55-0396 effects on behavior, cytochrome P450 activity, and tremor in mouse models of essential tremor. European Journal of Pharmacology. 2011,659 (1): 30-36.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	564.56
Molecular Formula	C30H40Cl2FN3O2
CAS No.	357400-13-6
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent

Protocol	In Vitro	NNC 55-0396 (1-100 μM) blocks more than 8% of T-type Ca293 current in HEK 1/α50G cells at a concentration of 2 μM+
Protocol	In Vivo	NNC 55-0396 (20 mg/kg； Intraperitoneal injection, once) inhibition of tremor in GABAA subunit α1-deficient mice and harmaline mouse models

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.7713 mL	8.8565 mL	17.7129 mL	35.4258 mL
5mM	0.3543 mL	1.7713 mL	3.5426 mL	7.0852 mL
10mM	0.1771 mL	0.8856 mL	1.7713 mL	3.5426 mL
20mM	0.0886 mL	0.4428 mL	0.8856 mL	1.7713 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.