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| Size | Price | Stock |
|---|---|---|
| 250mg | $750 | Check With Us |
| 500mg | $1070 | Check With Us |
| 1g | $1605 | Check With Us |
: Cat #: V2716 CAS #: 214358-33-5 Purity ≥ 98%
Description: 1400W (1400-W) is a slow, tight binding, potent, and highly selective inhibitor of inducible nitric-oxide synthase (iNOS) with Ki values of 7 nM. The slow onset of inhibition by 1400W showed saturation kinetics with a maximal rate constant of 0.028 s-1 and a binding constant of 2.0 microM.
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| Molecular Weight (MW) | 250.17 |
|---|---|
| Molecular Formula | C10H15N3.2HCl |
| CAS No. | 214358-33-5 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO:35 mg/mL (139.9 mM)r |
| Water:35 mg/mL (139.9 mM)r | |
| Ethanol:35 mg/mL (139.9 mM) | |
| SMILES Code | CC(NCC1=CC=CC(CN)=C1)=N.[H]Cl.[H]Cl |
| Synonyms | 1400W HCl; 1400-W hydrochloride; 1400 W HCl |
| Protocol | In Vitro | 1400W is a slow, tight binding inhibitor of human inducible nitric- oxide synthase (iNOS). The slow onset of inhibition by 1400W shows saturation kinetics with a maximal rate constant of 0.028 s-1 and a binding constant of 2.0 μM. Inhibition is dependent on the cofactor NADPH. 1400W is at least 5000-fold selective for iNOS versus eNOS. In contrast, inhibition of human neuronal NOS and endothelial NOS (eNOS) is relatively weaker, rapidly reversible, and competitive with L-arginine, with Ki values of 2 μM and 50 μM, respectively. |
|---|---|---|
| In Vivo | 1400W potently (ED50=0.3 mg/kg) reduces the delayed vascular injury in rats attributable to LPS-induced iNOS but fails to exacerbate acute vascular leakage when given concurrently with LPS. |
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.9973 mL | 19.9864 mL | 39.9728 mL | 79.9456 mL |
| 5mM | 0.7995 mL | 3.9973 mL | 7.9946 mL | 15.9891 mL |
| 10mM | 0.3997 mL | 1.9986 mL | 3.9973 mL | 7.9946 mL |
| 20mM | 0.1999 mL | 0.9993 mL | 1.9986 mL | 3.9973 mL |
This equation is commonly abbreviated as: C1 V1 = C2 V2
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.