Amifampridine phosphate

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
1g	$250	To Be Confirmed
2g	$400	To Be Confirmed
5g	$600	To Be Confirmed

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Cat #: V3862 CAS #: 446254-47-3 Purity ≥ 98%

Description: Amifampridine phosphate (trade name: Firdapse; pyridine-3,4-diamine, 3,4-diaminopyridine, 3,4-DAP) is the phosphate salt of Amifampridine, which is an FDA approved drug used mainly in the treatment of a number of rare muscle diseases such as Lambert-Eaton myasthenic syndrome (LEMS) in adults (It gained US approval in November 2018).

References: Ojala KS, et al. A high-affinity, partial antagonist effect of 3,4-diaminopyridine mediates action potential broadening and enhancement of transmitter release at NMJs. J Biol Chem. 2021 Jan-Jun;296:100302.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	207.13
Molecular Formula	C5H10N3O4P
CAS No.	446254-47-3
Storage	-20℃ for 3 years in powder formrr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: ≥ 100 mg/mLrr
	Water: N/Arr
	Ethanol: N/A
SMILES Code	NC1=C(N)C=NC=C1.O=P(O)(O)O
Synonyms	3,6-DAP phosphate; 3,4-Diaminopyridine phosphate; BRN-0110232 phosphate; BRN 0110232; BRN0110232; NSC 521760; NSC-521760; NSC521760 phosphate; SC10; Trade name: Firdapse.

Protocol	In Vitro	Amifampridine phosphate (1.5 μM) significantly reduces Kv3.3 and Kv3.4 currents by about 10% in HEK293T cells, has no effect on Cav2.1 or Cav1.2 current

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	4.8279 mL	24.1394 mL	48.2789 mL	96.5577 mL
5mM	0.9656 mL	4.8279 mL	9.6558 mL	19.3115 mL
10mM	0.4828 mL	2.4139 mL	4.8279 mL	9.6558 mL
20mM	0.2414 mL	1.2070 mL	2.4139 mL	4.8279 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.