Disodium (R)-2-Hydroxyglutarate
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 100mg | $195 | In Stock |
| 250mg | $380 | In Stock |
| 500mg | $570 | In Stock |
: Cat #: V1889 CAS #: 103404-90-6 Purity ≥ 98%
Description: Disodium (R)-2-Hydroxyglutarate (also known as D-2-HGA, or 2-HG) is a potent and competitive inhibitor of α-ketoglutarate (α-KG)-dependent dioxygenases with Ki value of 0.628 mM.
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| Molecular Weight (MW) | 192.08 |
|---|---|
| Molecular Formula | C5H6Na2O5 |
| CAS No. | 103404-90-6 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: <1 mg/mLr |
| Water: 38 mg/mL (197.83 mM)r | |
| Ethanol: <1 mg/mL | |
| SMILES Code | O=C([O-])[C@H](O)CCC([O-])=O.[Na+].[Na+] |
| Synonyms | D-alpha-Hydroxyglutaric acid disodium salt; Disodium (R)-2-hydroxyglutarate; D-2-HGA; 2R-hydroxy-pentanedioic acid, disodium salt |
| Protocol | In Vitro | D-α-Hydroxyglutaric acid is a weak inhibitor of TET hydroxylases. In the presence of 0.1 mM of α-KG, addition of 10 mM D-α-Hydroxyglutaric acid results in a partial (33%) inhibition of TET2 and addition of 50 mM D-α-Hydroxyglutaric acid results in more inhibition (83%) of TET2. D-α-Hydroxyglutaric acid exhibits a less pronounced inhibitory effect toward TET1. A partial inhibition of KDM7A toward both H3K9me2 and H3K27me2 peptides is observed in the presence of 50 mM D-2-HG and 100 μM α-ketoglutarate (α-KG). Addition of 300 μM α-KG is capable of reversing the inhibition of Caenorhabditis elegans KDM7A (CeKDM7A) by 50 mM D-2-HG, indicating that D-2-HG is a weak competitive inhibitor against α-KG toward the CeKDM7A demethylase. D-α-Hydroxyglutaric acid ((R)-2-hydroxyglutarate) accumulates in human cancers carrying neomorphic mutations in the isocitrate dehydrogenase (IDH) 1 and 2 genes. |
|---|---|---|
| In Vivo | D-α-Hydroxyglutaric acid strongly inhibits glucose utilization, CO2 production and the respiratory chain in rat cerebral cortex and human skeletal muscle, as well as in submitochondrial particles from bovine heart, suggesting an impairment of the aerobic metabolism. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 5.2062 mL | 26.0308 mL | 52.0616 mL | 104.1233 mL |
| 5mM | 1.0412 mL | 5.2062 mL | 10.4123 mL | 20.8247 mL |
| 10mM | 0.5206 mL | 2.6031 mL | 5.2062 mL | 10.4123 mL |
| 20mM | 0.2603 mL | 1.3015 mL | 2.6031 mL | 5.2062 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
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Volume
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Molecular Weight*
The dilution calculator equation
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Volume(start)
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.