EBE-A22 | CAS 229476-53-3

EBE-A22

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
250mg	$950	Check With Us
500mg	$1650	Check With Us
1g	$2475	Check With Us

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Cat #: V4000 CAS #: 229476-53-3 Purity ≥ 98%

Description: EBE-A22 is a N-methyl derivative of PD-153035 which exhibits a very high affinity and selectivity for the epidermal growth factor receptor tyrosine kinase (EGF-R TK) and shows a remarkable cytotoxicity against several types of tumor cell lines.

References: Goossens JF, et al. DNA interaction of the tyrosine protein kinase inhibitor PD153035 and its N-methyl analogue. Biochemistry. 2001 Apr 17;40(15):4663-71.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	374.24
Molecular Formula	C17H16BrN3O2
CAS No.	229476-53-3
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 10mMr
	Water: N/Ar
	Ethanol: N/A
SMILES Code	CN(C1=CC=CC(Br)=C1)C2=C3C=C(OC)C(OC)=CC3=NC=N2
Synonyms	EBE-A22; EBE A22; EBEA22; N-methyl derivative of PD153035

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.6721 mL	13.3604 mL	26.7208 mL	53.4416 mL
5mM	0.5344 mL	2.6721 mL	5.3442 mL	10.6883 mL
10mM	0.2672 mL	1.3360 mL	2.6721 mL	5.3442 mL
20mM	0.1336 mL	0.6680 mL	1.3360 mL	2.6721 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.