Spebrutinib besylate
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 500mg | $750 | To Be Confirmed |
| 1g | $1250 | To Be Confirmed |
| 5g | $3165 | To Be Confirmed |
: Cat #: V4768 CAS #: 1360053-81-1 Purity ≥ 98%
Description: Spebrutinib (formerly known as AVL-292 and CC-292) is a novel, potent, covalent/irreversible, orally bioactive, and highly selective Btk kinase inhibitor with an IC50 of 0.5 nM.
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| Molecular Weight (MW) | 581.62 |
|---|---|
| Molecular Formula | C28H28FN5O6S |
| CAS No. | 1360053-81-1 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| SMILES Code | COCCOC1=CC=C(NC2=NC=C(F)C(NC3=CC=CC(NC(C=C)=O)=C3)=N2)C=C1.O=S(C4=CC=CC=C4)(O)=O |
| Synonyms | AVL292; AVL-292; AVL 292; CC292; CC-292; CC 292 |
| Protocol | In Vitro | Spebrutinib (CC-292) is a covalent, highly selective, orally active inhibitor of Btk with IC50 value of 0.5 nM. Spebrutinib also less potently inhibits Yes, c-Src, Brk, Lyn, and Fyn with IC50s of 723 nM, 1.729 μM, 2.43 μM, 4.4 μM, and 7.15 μM, rspectively. Extensive analysis has revealed that the EC50 of Btk occupancy from a Spebrutinib dose-response in Ramos cells (EC50=6 nM) correlated directly with the cellular EC50 of Btk kinase inhibition with Spebrutinib (EC50=8 nM). Furthermore, the concentration at which Spebrutinib inhibits 90% of Btk activity in Ramos cells is 35 nM while the concentration of Spebrutinib required for 90% occupancy of Btk is 39 nM. |
|---|
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 1.7193 mL | 8.5967 mL | 17.1934 mL | 34.3867 mL |
| 5mM | 0.3439 mL | 1.7193 mL | 3.4387 mL | 6.8773 mL |
| 10mM | 0.1719 mL | 0.8597 mL | 1.7193 mL | 3.4387 mL |
| 20mM | 0.0860 mL | 0.4298 mL | 0.8597 mL | 1.7193 mL |
The molarity calculator equation
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The dilution calculator equation
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.