E-64 HCl | CAS 66701-25-5 (free base)

E-64 HCl

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
250mg	$750	Check With Us
500mg	$1150	Check With Us
1g	$1725	Check With Us

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Cat #: V3413 CAS #: 66701-25-5 (free base) Purity ≥ 98%

Description: E-64 HCl, the hydrochloride salt form of E-64, which is isolated from cultures of Aspergillus, is a novel, potent, irreversible and selective cysteine protease inhibitor with IC50 of 9 nM for papain.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	393.86
Molecular Formula	C15H28CLN5O5
CAS No.	66701-25-5 (free base)
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 71 mg/mL (198.7 mM)r
	Water: <1 mg/mLr
	Ethanol: 11 mg/mL (30.8 mM)
SMILES Code	O=C([C@@H]1[C@@H](C(O)=O)O1)N[C@H](C(NCCCCNC(N)=N)=O)CC(C)C.[H]Cl
Synonyms	E-64 HCl; E 64 HCl; E64 HCl

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.5390 mL	12.6949 mL	25.3897 mL	50.7795 mL
5mM	0.5078 mL	2.5390 mL	5.0779 mL	10.1559 mL
10mM	0.2539 mL	1.2695 mL	2.5390 mL	5.0779 mL
20mM	0.1269 mL	0.6347 mL	1.2695 mL	2.5390 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.