Anisomycin (NSC-76712, AI 3-50846, Flagecidin, Wuningmeisu C)
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 500mg | $450 | In Stock |
| 1g | $650 | In Stock |
| 5g | $1645 | In Stock |
: Cat #: V0475 CAS #: 22862-76-6 Purity ≥ 98%
Description: Anisomycin (Wuningmeisu C, NSC76712, AI3-50846, Flagecidin), a naturally occuring bacterial antibiotic isolated from Streptomyces griseolus, is a novel, potent and specific activator (agonist) of JNK (c-Jun N-terminal Kinase) with potential antineoplastic activity.
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| Molecular Weight (MW) | 265.3 |
|---|---|
| Molecular Formula | C14H19NO4 |
| CAS No. | 22862-76-6 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 41 mg/mL (154.5 mM)r |
| Water:<1 mg/mLr | |
| Ethanol: 17 mg/mL warmed (64.1 mM) | |
| Solubility In Vivo | 2% DMSO+corn oil: 5mg/mL |
| SMILES Code | O[C@@H]1[C@@H](OC(C)=O)[C@@H](CC2=CC=C(OC)C=C2)NC1 |
| Synonyms | Wuningmeisu C; NSC 76712; AI3 50846; NSC-76712; AI-350846; NSC76712; AI350846; Flagecidin; |
| Protocol | In Vitro | To examine whether JNK has a core role in colistin-induced neurotoxicity in PC-12 cells, an SP600125 (a highly selective inhibitor of JNK) and Anisomycin (a potent activator) are used in this study. In order to select an appropriate concentration, PC-12 cells are treated with a range of SP600125 (0-80 μM) and Anisomycin (0-20 μM) respectively for 24 h. The results show that the cells viability significantly decreases by SP600125 treatment in a concentration-dependent manner, observed at the concentrations greater than 20 μM (p<0.01). Similarly the cells viability is inhibited by Anisomycin treatment (≥8 μM) (p<0.05) . |
|---|---|---|
| In Vivo | Anisomycin (60 mg/kg; for 4-week continuous intravenous administration) significantly decreases mouse body weight in a dose-related manner, compared with the control group. Anisomycin (15 mg/kg; for 4-week continuous intravenous administration) slightly and transiently decreases the mouse body weight. There is no significant difference of the mouse body weight in 5 mg/kg group. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.7693 mL | 18.8466 mL | 37.6932 mL | 75.3864 mL |
| 5mM | 0.7539 mL | 3.7693 mL | 7.5386 mL | 15.0773 mL |
| 10mM | 0.3769 mL | 1.8847 mL | 3.7693 mL | 7.5386 mL |
| 20mM | 0.1885 mL | 0.9423 mL | 1.8847 mL | 3.7693 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
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Volume
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The dilution calculator equation
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
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Average weight of animals g
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Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.