Ascomycin (FK520)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
500mg	$290	Check With Us
1g	$480	Check With Us
5g	$810	Check With Us

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Cat #: V1936 CAS #: 104987-12-4 Purity ≥ 98%

Description: Ascomycin (also known as FK520, FR 900520, Immunomycin), a natural product isolated from Streptomyces, is an ethyl analog of tacrolimus (FK506) and a neutral macrolide immunosuppressant, which prevents rejection after an organ transplant.

References: Hatanaka H, et al. FR-900520 and FR-900523, novel immunosuppressants isolated from a Streptomyces. II. Fermentation, isolation and physico-chemical and biological characteristics. J Antibiot (Tokyo). 1988 Nov;41(11):1592-601.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	792.01
Molecular Formula	C43H69NO12
CAS No.	104987-12-4
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 100 mg/mL (126.3 mM)r
	Water: <1 mg/mLr
	Ethanol: 100 mg/mL (126.3 mM)
SMILES Code	O=C([C@@](CCCC1)([H])N1C(C([C@@]2(O)[C@H](C)C[C@H](OC)[C@@](O2)([H])[C@@H](OC)C[C@@H](C)C/C(C)=C/[C@H]3CC)=O)=O)O[C@H](/C(C)=C/[C@H]4C[C@@H](OC)[C@H](O)CC4)[C@H](C)[C@@H](O)CC3=O
Synonyms	FR 900520, FK-520; FK-520; FK-520; FR900520; FR-900520; FR-900520; L 683590; L-683590; L683590; Changchuanmycin; Immunomycin

Protocol	In Vitro	Ascomycin (FR-900520) suppresses immune response in vitro with an IC50 value of 0.55 nM for mouse mixed lymphocyte reaction.
Protocol	In Vivo	Ascomycin (FR-900520; i.m.; 0.32-32 mg/kg; 5 days a week; for 2 weeks) clearly prolongs skin allograft survival in rats.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.2626 mL	6.3131 mL	12.6261 mL	25.2522 mL
5mM	0.2525 mL	1.2626 mL	2.5252 mL	5.0504 mL
10mM	0.1263 mL	0.6313 mL	1.2626 mL	2.5252 mL
20mM	0.0631 mL	0.3157 mL	0.6313 mL	1.2626 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.