Puromycin Aminonucleoside (NSC-3056)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
500mg	$850	To Be Confirmed
1g	$1550	To Be Confirmed
5g	$3925	To Be Confirmed

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Cat #: V5081 CAS #: 58-60-6 Purity ≥ 98%

Description: Puromycin aminonucleoside (formerly known as NSC 3056; PAN, NSC-3056), the aminonucleoside portion of the antibiotic puromycin, is commonly used in nephrosis animal models as well as in the study of human glomerular disease, and glomerular function and morphology.

References: Lacalle RA, et al. Cloning of the complete biosynthetic gene cluster for an aminonucleoside antibiotic, puromycin, and its regulated expression in heterologous hosts. EMBO J. 1992 Feb;11(2):785-92.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	294.31
Molecular Formula	C12H18N6O3
CAS No.	58-60-6
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
SMILES Code	O[C@H]([C@@H]1N)[C@@H](O[C@@H]1CO)N2C(N=CN=C3N(C)C)=C3N=C2
Synonyms	Puromycin Aminonucleoside; ARDMA; NSC 3056; SAN; Stylomycin aminonucleoside

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.3978 mL	16.9889 mL	33.9778 mL	67.9556 mL
5mM	0.6796 mL	3.3978 mL	6.7956 mL	13.5911 mL
10mM	0.3398 mL	1.6989 mL	3.3978 mL	6.7956 mL
20mM	0.1699 mL	0.8494 mL	1.6989 mL	3.3978 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.