Loxistatin Acid (E-64C; NSC694279; EP475)
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 100mg | $1550 | To Be Confirmed |
| 250mg | $2350 | To Be Confirmed |
| 500mg | $3525 | To Be Confirmed |
: Cat #: V0700 CAS #: 76684-89-4 Purity ≥ 98%
Description: Loxistatin Acid (formerly also known as E-64C; NSC-694279; EP-475), an analog of E-64, is a novel, potent, irreversible and membrane-permeable cysteine protease inhibitor with important biological activity.
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- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 314.38 |
|---|---|
| Molecular Formula | C15H26N2O5 |
| CAS No. | 76684-89-4 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 62 mg/mL (197.2 mM)r |
| Water: 2 mg/mL (6.4 mM)r | |
| Ethanol: 62 mg/mL (197.2 mM) | |
| Solubility In Vivo | Saline: 2mg/mL |
| SMILES Code | OC([C@@H]1[C@@H](C(N[C@H](C(NCCC(C)C)=O)CC(C)C)=O)O1)=O |
| Synonyms | NSC-694279; EP-475; E64-C; NSC 694279; EP 475; E64 C; NSC694279; EP475; E64C; Loxistatin Acid; |
| Protocol | In Vitro | E-64c, a derivative of naturally occurring epoxide inhibitor of cysteine proteases, with papain; especially with regard to the hydrogen bonding and hydrophobic interactions of the ligands with conserved residues in the catalytic binding site. |
|---|---|---|
| In Vivo | The t-1/2 of plasma E-64c is 0.48 hours. The hemodynamic effects of E-64c are absent at this dose. Using two way analysis of variance, the effects of reperfusion (p=0.0016) or E-64c (p=0.0226) per se on infarct size are significant. In comparing Group A with Group B and Group C with Group D, the depletion of CPK in the E-64c treated groups (Groups A and C) is slightly less than in the vehicle-injected groups (Groups B and D). The insufficient effect of E-64c alone may be explained by the early administration and relatively short t-1/2. Since the effectiveness of NCO-700 has been established,6),7) our findings might indicate a small but beneficial effect of E-64c on infarct size and CPK content. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.1809 mL | 15.9043 mL | 31.8086 mL | 63.6173 mL |
| 5mM | 0.6362 mL | 3.1809 mL | 6.3617 mL | 12.7235 mL |
| 10mM | 0.3181 mL | 1.5904 mL | 3.1809 mL | 6.3617 mL |
| 20mM | 0.1590 mL | 0.7952 mL | 1.5904 mL | 3.1809 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
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Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.