BRD9539
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $980 | Check With Us |
| 500mg | $1420 | Check With Us |
| 1g | $2130 | Check With Us |
: Cat #: V3455 CAS #: 1374601-41-8 Purity ≥ 98%
Description: BRD9539 is a potent and selective inhibitor that inhibits G9a activity with an IC50 of 6.3 μM, it also inhibits euchromatin histone methyltransferase 2 (EHMT2).
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| Molecular Weight (MW) | 399.44 |
|---|---|
| Molecular Formula | C24H21N3O3 |
| CAS No. | 1374601-41-8 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| SMILES Code | O=C(C1=CC=C(N(CCCC2=CC=CC=C2)C(NC(C3=CC=CC=C3)=O)=N4)C4=C1)O |
| Synonyms | BRD9539; BRD 9539; BRD-9539. |
| Protocol | In Vitro | BRD9539 is a more potent biochemical inhibitor than its methyl-ester analogue BRD4770, with 20% remaining G9a activity compared to 45% of BRD4770 at screening concentration. However, BRD9539 has no activity in cell-based assays, presumably due to impaired cell permeability compared to that of BRD4770. In addition, the activities of 16 other chromatin-modifying enzymes and 100 kinases involved in cell-cycle regulation and cancer cell biology are tested for activity in the presence of 5 or 10 μM BRD9539; no activity is seen in any of these assays. BRD9539 decreases ATP levels in a dose-dependent manner in HeLa cells. |
|---|
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.5035 mL | 12.5175 mL | 25.0350 mL | 50.0701 mL |
| 5mM | 0.5007 mL | 2.5035 mL | 5.0070 mL | 10.0140 mL |
| 10mM | 0.2504 mL | 1.2518 mL | 2.5035 mL | 5.0070 mL |
| 20mM | 0.1252 mL | 0.6259 mL | 1.2518 mL | 2.5035 mL |
The molarity calculator equation
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The dilution calculator equation
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.