NCGC00244536
This product is for research use only, not for human use. We do not sell to patients.
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| Size | Price | Stock |
|---|---|---|
| 100mg | $1430 | Check With Us |
| 250mg | $2290 | Check With Us |
| 500mg | $3435 | Check With Us |
: Cat #: V3301 CAS #: 2003260-55-5 Purity ≥ 98%
Description: NCGC00244536 (also known as KDM4B Inhibitor B3; KDM4B-IN-B3) is a novel and potent inhibitor of the Histone lysine demethylase KDM4 with an IC50 of 10 nM.
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- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 382.46 |
|---|---|
| Molecular Formula | C25H22N2O2 |
| CAS No. | 2003260-55-5 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: ≥100mg/mLr |
| Water: N/Ar | |
| Ethanol: N/A | |
| SMILES Code | O=C(NCCCC1=CC=CC=C1)C2=CC=CC(C3=CC(O)=C4N=CC=CC4=C3)=C2 |
| Synonyms | KDM4B-IN-B3; KDM4B IN B3; KDM4BINB3; KDM4B Inhibitor B3; KDM4B Inhibitor-B3; KDM4B Inhibitor B-3 |
| Protocol | In Vitro | NCGC00244536 displays high selectivity for the fast-growing AR-negative PC3 cells (IC50=40 nM) and over 100-fold selectivity against the immortalized prostate epithelial cell lines PrEC1 and PrEC4. NCGC00244536 effectively inhibits AR-positive cell lines, including LNCaP and VCaP, with IC50s in the sub-micromolar range, and abolishes androgen-stimulated LNCaP cell growth. NCGC00244536 is also potent in inhibiting the growth of other cancer cell lines, including the breast cancer cell lines MDA-MB2 and MCF-7, with micromolar IC50s. |
|---|---|---|
| In Vivo | Treatment with NCGC00244536 results in significant inhibition of tumor growth and animals do not exhibit any major toxicity and appear to be normal. Histological data clearly indicate that NCGC00244536-treated tumors have significant amount of cell apoptosis, necrosis, and fibrosis. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.6147 mL | 13.0733 mL | 26.1465 mL | 52.2931 mL |
| 5mM | 0.5229 mL | 2.6147 mL | 5.2293 mL | 10.4586 mL |
| 10mM | 0.2615 mL | 1.3073 mL | 2.6147 mL | 5.2293 mL |
| 20mM | 0.1307 mL | 0.6537 mL | 1.3073 mL | 2.6147 mL |
The molarity calculator equation
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The dilution calculator equation
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.