Marizomib (Salinosporamide A; NPI-0052)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
100mg	$12335	To Be Confirmed
200mg	$18500	To Be Confirmed
500mg	$27750	To Be Confirmed

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Cat #: V2138 CAS #: 437742-34-2 Purity ≥ 98%

Description: Marizomib (formerly also known as ML-858, NPI-0052 and Salinosporamide A), a naturally-occurring salinosporamide extracted from the marine actinomycete Salinospora tropica, is a potent and selective 20S proteasome inhibitor (IC50 = 1.3 nM) with potential antineoplastic activity.

References: Di K, et al. Marizomib activity as a single agent in malignant gliomas: ability to cross the blood-brainbarrier. Neuro Oncol. 2016 Jun;18(6):840-8.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	313.78
Molecular Formula	C15H20ClNO4
CAS No.	437742-34-2
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
SMILES Code	C[C@]12[C@H](C(=O)N[C@]1(C(=O)O2)[C@H]([C@H]3CCCC=C3)O)CCCl
Synonyms	(-)-Salinosporamide A; Salinosporamide A; ML 858; ML-858; ML858; NPI 0052; NPI-0052; NPI0052; Marizomib;

Protocol	In Vitro	Marizomib (24 hours; 60 nM) induces apoptosis and caspase-3 activation in glioma cells. Marizomib (Salinosporamide A) (0.1-10000 nM; 72 hours) effectively reduces survival of D-54 and U-251 cells in a dose-dependent manner. The IC50s are ∼52 nM for U-251 and ∼20 nM for D-54.
Protocol	In Vivo	Marizomib (Salinosporamide A) (0.15 mg/kg; i.v; twice a week for three weeks) significantly decreases tumor growth, and is not associated with any toxicity.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.1869 mL	15.9347 mL	31.8695 mL	63.7389 mL
5mM	0.6374 mL	3.1869 mL	6.3739 mL	12.7478 mL
10mM	0.3187 mL	1.5935 mL	3.1869 mL	6.3739 mL
20mM	0.1593 mL	0.7967 mL	1.5935 mL	3.1869 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.