DASA-58
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 100mg | $960 | Check With Us |
| 250mg | $1750 | Check With Us |
| 500mg | $2625 | Check With Us |
: Cat #: V1874 CAS #: 1203494-49-8 Purity ≥ 98%
Description: DASA-58 is a potent and selective activator of pyruvate kinase M2 (PKM2) with an AC90 value of 680 nM, and an AC50 value of 38 nM.
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| Molecular Weight (MW) | 453.53 |
|---|---|
| Molecular Formula | C19H23N3O6S2 |
| CAS No. | 1203494-49-8 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 91 mg/mL (200.6 mM)r |
| Water: <1 mg/mLr | |
| Ethanol: <1 mg/mL | |
| SMILES Code | O=S(N1CCN(S(C2=CC=C(OCCO3)C3=C2)(=O)=O)CCC1)(C4=CC(N)=CC=C4)=O |
| Synonyms | ML-203; ML 203; ML203; DASA-58; DASA 58; DASA58; NCGC00185916; NCGC-00185916; NCGC 00185916 |
| Protocol | In Vitro | DASA-58 (15 μM; 0-72 h) leads to depletion in TXNIP levels independent of AMPK and ER signaling, and not through enhanced proteasomal degradation but rather depleted upstream glycolytic intermediates. DASA-58 (15 μM; 0-72 h) not rescues TXNIP levels in any combintion and mitochondrial inhibitors enhance PKM2 effects on activating AMPK signaling (T172 phosphorylation of AMPK). DASA-58 (15 μM, 30 μM; 0-72 h) affects respiration levels in BCa cells without an indication of mitochondrial damage. DASA-58 (30 μM, 60 μM; 0-72 h) enhances extracellular acidification and lactate levels in BCa cell lines and induce extracellular acidification levels in prostate cancer cell lines. DASA-58 (15 μM; 24 h, 72 h) enhances pyruvate kinase activity in breast cancer cells without a clear effect on proliferation. DASA-58 (15 μM; 2 h) potentiates the antitumor effects of other metabolic stressors. |
|---|
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.2049 mL | 11.0246 mL | 22.0493 mL | 44.0985 mL |
| 5mM | 0.4410 mL | 2.2049 mL | 4.4099 mL | 8.8197 mL |
| 10mM | 0.2205 mL | 1.1025 mL | 2.2049 mL | 4.4099 mL |
| 20mM | 0.1102 mL | 0.5512 mL | 1.1025 mL | 2.2049 mL |
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The dilution calculator equation
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Step One: Enter information below
Dosage mg/kg
Average weight of animals g
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Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
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mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.