H 89 2HCl
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 500mg | $700 | Check With Us |
| 1g | $1150 | Check With Us |
| 5g | $1725 | Check With Us |
: Cat #: V0243 CAS #: 130964-39-5 Purity ≥ 98%
Description: H 89 2HCl, the dihydrochloride salt of H 89 (H-89), is a novel, potent and cAMP-dependent inhibitor of PKA (protein kinase A) with the potential to attenuates prominent behavioral signs of morphine withdrawal in morphine-dependent mice.
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| Molecular Weight (MW) | 519.28 |
|---|---|
| Molecular Formula | C20H20BrN3O2S.2HCl |
| CAS No. | 130964-39-5 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 104 mg/mL (200.3 mM)r |
| Water: 6 mg/mL (11.6 mM)r | |
| Ethanol: <1 mg/mL | |
| Solubility In Vivo | 1% DMSO+30% polyethylene glycol+1% Tween 80: 30mg/mL |
| SMILES Code | O=S(C1=CC=CC2=C1C=CN=C2)(NCCNC/C=C/C3=CC=C(Br)C=C3)=O.[H]Cl.[H]Cl |
| Synonyms | H-89; H-89 Dihydrochloride; H89; H 89 HCl |
| Protocol | In Vitro | H-89 inhibits protein kinase A, in competitive fashion against ATP. H-89 causes a dose-dependent inhibition of the forskolin-induced protein phosphorylation, with no decrease in intracellular cyclic AMP levels in PC12D cells. H-89 significantly inhibits the forskolin-induced neurite outgrowth from PC12D cells. H-89 (30 μM) inhibits significantly cAMP-dependent histone IIb phosphorylation activity in PC12D cell lysates. |
|---|
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 1.9257 mL | 9.6287 mL | 19.2574 mL | 38.5149 mL |
| 5mM | 0.3851 mL | 1.9257 mL | 3.8515 mL | 7.7030 mL |
| 10mM | 0.1926 mL | 0.9629 mL | 1.9257 mL | 3.8515 mL |
| 20mM | 0.0963 mL | 0.4814 mL | 0.9629 mL | 1.9257 mL |
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Step One: Enter information below
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Step Two: Enter the in vivo formulation
%DMSO
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%Tween 80
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%ddH2O
Calculation Results:
Working concentration:
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Method for preparing DMSO master liquid:
mg
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,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.