AS-604850
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $995 | Check With Us |
| 500mg | $1690 | Check With Us |
| 1g | $2535 | Check With Us |
: Cat #: V0134 CAS #: 648449-76-7 Purity ≥ 98%
Description: AS-604850 is a novel, potent, selective, and ATP-competitive inhibitor of PI3Kγ (phosphatidylinositol 3-kinase γ) with IC50 of 250 nM and a Ki of 180 nM.
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- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 285.22 |
|---|---|
| Molecular Formula | C11H5F2NO4S |
| CAS No. | 648449-76-7 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 57 mg/mL (199.84 mM)r |
| Water:<1 mg/mLr | |
| Ethanol: 5 mg/mL (17.53 mM) | |
| Solubility In Vivo | 0.5% CMC+0.25% Tween 80: 30mg/mL |
| SMILES Code | O=C(NC/1=O)SC1=C/C2=CC=C(OC(F)(F)O3)C3=C2 |
| Synonyms | AS604850; AS 604850; AS-604850 |
| Protocol | In Vitro | AS-604850 (0-30 μM; 15 minutes; primary monocytes from Pik3cg+/+ mice) treatment inhibits MCP-1-mediated phosphorylation of PKB and its downstream substrates GSK3α andβ in a concentration-dependent manner. MCP-1-induced phosphorylation of p44/42 ERK (ERK1/2) MAPKs is also reduced, in a concentration dependent manner in primary monocytes from Pik3cg+/+ mice. AS-604850 blocks PKB phosphorylation induced by MCP-1 and has little or no effect after stimulation with CSF-1 in in primary monocytes from Pik3cg+/+ or Pik3cg–/– mice. AS-604850 inhibits C5a-mediated PKB phosphorylation with an IC50 of 10 μM in RAW264 mouse macrophages. |
|---|---|---|
| In Vivo | AS-604850 (10-100 mg/kg; oral administration; for 4.5 or 4.25 hours; Balb/C or C3H mice) treatment reduces RANTES-induced peritoneal neutrophil recruitment with an ED50 value of 42.4 mg/kg. In the thioglycollate-induced peritonitis model, oral administration of 10 mg/kg AS-604850 results in a 31% reduction of neutrophil recruitment. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 3.5061 mL | 17.5303 mL | 35.0607 mL | 70.1213 mL |
| 5mM | 0.7012 mL | 3.5061 mL | 7.0121 mL | 14.0243 mL |
| 10mM | 0.3506 mL | 1.7530 mL | 3.5061 mL | 7.0121 mL |
| 20mM | 0.1753 mL | 0.8765 mL | 1.7530 mL | 3.5061 mL |
The molarity calculator equation
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The dilution calculator equation
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This equation is commonly abbreviated as: C1 V1 = C2 V2
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Step One: Enter information below
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Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
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mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.