Isatin (Indoline-2,3-dione)
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 100g | $250 | Check With Us |
| 200g | $400 | Check With Us |
| 500g | $675 | Check With Us |
: Cat #: V2630 CAS #: 91-56-5 Purity ≥ 98%
Description: Isatin (also named 2,3-Indolinedione) is an endogenous MAO inhibitor.
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- Related Biological Data
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| Molecular Weight (MW) | 147.13 |
|---|---|
| Molecular Formula | C8H5NO2 |
| CAS No. | 91-56-5 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 29 mg/mL (197.1 mM)r |
| Water: <1 mg/mLr | |
| Ethanol: 10 mg/mL (68 mM) | |
| SMILES Code | O=C1NC2=C(C=CC=C2)C1=O |
| Synonyms | 2,3-Indolinedione ;Isatin; 2,3-Dioxo-2,3-dihydroindole; Isatic acid lactam; Isotin |
| Protocol | In Vitro | In dopaminergic SH-SY5Y cells isatin (1-400 μM) induces cell death in dose- and time dependent manner. This death occurred as a continuum of survival, apoptosis and necrosis. |
|---|---|---|
| In Vivo | A single dose of isatin (80 mg/kg) has a rapid effect on the serotonergic system in the hypothalamus. Isatin significantly increases 5-HT concentrations in the hypothalamus and cortex but did not significantly alter 5-HIAA concentrations. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 6.7967 mL | 33.9836 mL | 67.9671 mL | 135.9342 mL |
| 5mM | 1.3593 mL | 6.7967 mL | 13.5934 mL | 27.1868 mL |
| 10mM | 0.6797 mL | 3.3984 mL | 6.7967 mL | 13.5934 mL |
| 20mM | 0.3398 mL | 1.6992 mL | 3.3984 mL | 6.7967 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.