Leucovorin calcium hydrate

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
25g	$450	Check With Us
50g	$750	Check With Us
100g	$1125	Check With Us

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Cat #: V25456 CAS #: 6035-45-6 Purity ≥ 99%

Description: Leucovorin calcium, a reduced folic acid and an active metabolite of folic acid (also known as folinic acid and citrovorum factor), is a derivative of folic acid which can be used to increase levels of folic acid under conditions favoring folic acid inhibition.

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Physicochemical and Storage Information
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Stock Solution Preparation
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Molecular Weight (MW)	601.58
Molecular Formula	C20H31CaN7O12
CAS No.	6035-45-6
SMILES Code	O=C([O-])CC[C@@H](C([O-])=O)NC(C1=CC=C(NCC2N(C=O)C3=C(NC(N)=NC3=O)NC2)C=C1)=O.[H]O[H].[H]O[H].[H]O[H].[H]O[H].[H]O[H].[Ca+2]
Synonyms	Folinic acid calcium salt pentahydrate; Leucovorin calcium hydrate;

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.6623 mL	8.3114 mL	16.6229 mL	33.2458 mL
5mM	0.3325 mL	1.6623 mL	3.3246 mL	6.6492 mL
10mM	0.1662 mL	0.8311 mL	1.6623 mL	3.3246 mL
20mM	0.0831 mL	0.4156 mL	0.8311 mL	1.6623 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.