Colistin sulfate

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
10g	$350	Check With Us
25g	$500	Check With Us
50g	$750	Check With Us

: [email protected] [email protected]

Cat #: V33375 CAS #: 1264-72-8 Purity ≥ 99%

Description: Colistin sulfate (Belcomycin; Polymixin E), originally isolated from B. polymyxa, is a polypeptide antibiotic and apoptosis inducer, also acts as a NADH quinone oxidoreductase inhibitor.

References: Hancock RE et al. Peptide antibiotics. Antimicrob Agents Chemother. 1999 Jun;43(6):1317-23.

Top Publications Citing Invivochem Products

Product Catalog 2022

Guide to Product Handling

Publications Citing InvivoChem Products

Product Promise

Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	1400.64
Molecular Formula	C52H98N16O13.2.5H2O4S
CAS No.	1264-72-8
SMILES Code	O=C(N[C@@H](CCN)C(N[C@H](CC(C)C)C(N[C@@H](CC(C)C)C(N[C@@H](CCN)C(N[C@H]1CCN)=O)=O)=O)=O)[C@@H](NC([C@H](CCN)NC([C@@H](NC([C@H](CCN)NC(CCCC[C@H](C)CC)=O)=O)[C@@H](C)O)=O)=O)CCNC([C@H]([C@@H](C)O)NC1=O)=O.OS(O)(=O)=O
Synonyms	Colistin sulfate; Belcomycin; Polymixin E;

Protocol	In Vitro	Colistins are bactericidal to gram-negative bacteria by a detergent-like mechanism. This mechanism involves interaction with lipopolysaccharides and phospholipids of the outer membrane and electrostatic interference with the outer membrane by competitively displacing divalent cations (calcium and magnesium) from the negatively charged phosphate groups of membrane lipids.
Protocol	In Vivo	High concentrations of colistin in rat ELF are achieved as a result of slow and sustained CMS conversion following i.t. instillation.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	0.7140 mL	3.5698 mL	7.1396 mL	14.2792 mL
5mM	0.1428 mL	0.7140 mL	1.4279 mL	2.8558 mL
10mM	0.0714 mL	0.3570 mL	0.7140 mL	1.4279 mL
20mM	0.0357 mL	0.1785 mL	0.3570 mL	0.7140 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.