Geneticin G418

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
50g	$900	Check With Us
100g	$1550	Check With Us
200mg	$2325	Check With Us

: [email protected] [email protected]

Cat #: V10522 CAS #: 108321-42-2 Purity ≥ 98%

Description: Geneticin G418 (G-418 disulfate) is a potent aminoglycoside antibiotic of the gentamicin B1 class, blocking polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.

Top Publications Citing Invivochem Products

Product Catalog 2022

Guide to Product Handling

Publications Citing InvivoChem Products

Product Promise

Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	594.63
Molecular Formula	C20H42N4O14S
CAS No.	108321-42-2
SMILES Code	O[C@@H]1[C@H](O[C@@H]2[C@@H](O)[C@H](O[C@H]3O[C@H](C(O)C)[C@@H](O)[C@H](O)[C@H]3N)[C@@H](N)C[C@H]2N)OC[C@](O)(C)[C@H]1NC.O=S(O)(O)=O
Synonyms	Geneticin G 418; Antibiotic G418; Geneticin G418; Geneticin G-418;

Protocol	In Vitro	Geneticin G418 (G-418 disulfate) is an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/mL. Resistance to G418 conferring by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APT 3' II is commonly used in laboratory research to select genetically engineered cells[1]. In general for bacteria and algae concentrations of 5 mg/L or less are used, for mammalian cells concentrations of approximately 400 mg/L are used for selection and 200 mg/L for maintenance. Resistant clones selection could require from 1 to up to 3 weeks[2].
Protocol	In Vivo	Geneticin G418 (G-418 disulfate) (40 and 80 mg/kg) for three consecutive days are sufficient to eliminate all nontransfected T. brucei brucei parasites from infected mice[3].

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.6817 mL	8.4086 mL	16.8172 mL	33.6344 mL
5mM	0.3363 mL	1.6817 mL	3.3634 mL	6.7269 mL
10mM	0.1682 mL	0.8409 mL	1.6817 mL	3.3634 mL
20mM	0.0841 mL	0.4204 mL	0.8409 mL	1.6817 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.