Sitafloxacin (DU-6859a)

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
250mg	$650	Check With Us
500mg	$900	Check With Us
1g	$1350	Check With Us

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Cat #: V21049 CAS #: 127254-12-0 Purity ≥ 98%

Description: Sitafloxacin Hydrate (DU-6859a), a new-generation, broad-spectrum and orally bioavailable fluoroquinolone class of antibiotic, is highly active against many Gram-positive, Gram-negative and anaerobic clinical isolates, including strains resistant to other fluoroquinolones, was recently approved in Japan for the treatment of respiratory and urinary tract infections.

References: Okumura R, et al. Dual-targeting properties of the 3-aminopyrrolidyl quinolones, DC-159a and sitafloxacin, against DNA gyrase and topoisomerase IV: contribution to reducing in vitro emergence of quinolone-resistant Streptococcus pneumoniae. J Antimicrob Chemother. 2008 Jul;62(1):98-104.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	409.81
Molecular Formula	C19H18ClF2N3O3
CAS No.	127254-12-0
SMILES Code	O=C(C1=CN([C@H]2[C@@H](F)C2)C3=C(C=C(F)C(N(C[C@H]4N)CC54CC5)=C3Cl)C1=O)O.O=C(C6=CN([C@H]7[C@@H](F)C7)C8=C(C=C(F)C(N(C[C@H]9N)CC%109CC%10)=C8Cl)C6=O)O.[H]O[H].[H]O[H].[H]O[H]
Synonyms	Sitafloxacin hydrate; Sitafloxacin anhydrous; Sitafloxacin; DU 6859; DU-6859; DU6859; DU 6859A; DU-6859A; DU6859A; Trade name: Gracevit.

Protocol	In Vitro	Sitafloxacin (DU6859a) shows inhibition for DNA gyrase and topoisomerase IV (TopoIV) with >IC50s of 4.38, 3.12 mg/L, respectively. Sitafloxacin (DU6859a) shows antibacterial activities for quinolone-susceptible strains of streptococcus pneumoniae with MIC of 0.03, 0.03 mg/L for EG 00093 and EG 00218 strain, respectively. Sitafloxacin (DU6859a) shows antibacterial activities with MIC of 0.03, 0.12, 0.06 mg/L for wild-type ATCC 49619, gyrA mutant SP39, parC mutant 1026523 streptococcus pneumoniae stran, respectively.
	In Vivo	Sitafloxacin (DU-6859a) (12.5-100 mg/kg; i.g.; daily for 4 weeks; BALB/c female mice) has antibacterial activity. M. ulcerans cells could be isolated from the inoculated footpads and there was no evidence of footpad swelling.
	Animal model	BALB/c female mice

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.4402 mL	12.2008 mL	24.4016 mL	48.8031 mL
5mM	0.4880 mL	2.4402 mL	4.8803 mL	9.7606 mL
10mM	0.2440 mL	1.2201 mL	2.4402 mL	4.8803 mL
20mM	0.1220 mL	0.6100 mL	1.2201 mL	2.4402 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.