MMAF-OMe | CAS 863971-12-4

MMAF-OMe

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Size	Price	Stock
100mg	$2025	Check With Us
200mg	$3025	Check With Us
500mg	$5000	Check With Us

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Cat #: V29041 CAS #: 863971-12-4 Purity ≥ 99%

Description: MMAF-OMe (Monomethyl auristatin F methyl ester-O-methyl), an auristatin-derived anticancer agent, is a novel and potent warhead used in ADC (antibody-drug conjugate). It acts as an inhibitor of tubulin polymerization.

References: [1]. Currier NV, et al. Targeted Drug Delivery with an Integrin-Binding Knottin-Fc-MMAF Conjugate Produced by Cell-Free Protein Synthesis. Mol Cancer Ther. 2016 Jun;15(6):1291-300

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Physicochemical and Storage Information
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Molecular Weight (MW)	745.99
Molecular Formula	C40H67N5O8
CAS No.	863971-12-4
Solubility In Vitro	DMSO : ≥ 100 mg/mL (134.05 mM)
SMILES Code	O=C(OC)[C@H](CC1=CC=CC=C1)NC([C@H](C)[C@H]([C@H]2N(C(C[C@@H](OC)[C@@H](N(C)C([C@@H](NC([C@@H](NC)C(C)C)=O)C(C)C)=O)[C@@H](C)CC)=O)CCC2)OC)=O
Synonyms	Monomethyl auristatin F methyl ester; MMAFOMe;

Protocol	In Vitro	2.5F-Fc and 2.5F-Fc-MMAF have similar IC50 values (6.9±1.1 vs. 8.3±1.3 nM, respectively), indicating that MMAF conjugation has negligible impact on integrin-binding affinity.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.3405 mL	6.7025 mL	13.4050 mL	26.8100 mL
5mM	0.2681 mL	1.3405 mL	2.6810 mL	5.3620 mL
10mM	0.1341 mL	0.6703 mL	1.3405 mL	2.6810 mL
20mM	0.0670 mL	0.3351 mL	0.6703 mL	1.3405 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.