Maytansinoid DM4

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
250mg	$1600	Check With Us
500mg	$2600	Check With Us
1g	$3900	Check With Us

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Cat #: V25076 CAS #: 796073-69-3 Purity ≥ 98%

Description: Maytansinoid DM4, a naturally occuring maytansine analogue, is a highly potent/cytotoxic antitubulin agent with anticancer properties. Maytansinoids have been shown to be able to disrupt microtubule function. Maytansinoid DM4 can be used for the preparation of antibody drug conjugates such as Anetumab ravtansine (BAY 94-9343) and Indatuximab ravtansine (BT062).

References: [1]. Tang R, et al. P-gp activity is a critical resistance factor against AVE9633 and DM4 cytotoxicity in leukaemia cell lines, but not a major mechanism of chemoresistance in cells from acute myeloid leukaemia patients. BMC Cancer. 2009 Jun 23;9:199.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	780.37
Molecular Formula	C38H54ClN3O10S
CAS No.	796073-69-3
Solubility In Vitro	DMSO : 100 mg/mL (128.14 mM; Need ultrasonic)
SMILES Code	C/C(CC1=CC(OC)=C(Cl)C(N(C(C[C@]([C@]2(C)O[C@H]2[C@@H]3C)([H])OC([C@@H](N(C(CCC(C)(C)S)=O)C)C)=O)=O)C)=C1)=CC=C[C@@H](OC)[C@](NC4=O)(C[C@]3([H])O4)O
Synonyms	Maytansinoid DM4; Soravtansine; Ravtansine; DM4;

Protocol	In Vitro	DM4, a structural analogue of maytansine, is a new thiol-containing and potent maytansinoid. DM4 is a cytotoxic maytansinoid drug. It is synthesized in order to link maytansinoids to antibodies via disulfide bonds. Maytansinoids inhibit tubulin polymerization and microtubule assembly and enhance microtubule destabilization, so there is potent suppression of microtubule dynamics resulting in a mitotic block and subsequent apoptotic cell death.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.2814 mL	6.4072 mL	12.8144 mL	25.6289 mL
5mM	0.2563 mL	1.2814 mL	2.5629 mL	5.1258 mL
10mM	0.1281 mL	0.6407 mL	1.2814 mL	2.5629 mL
20mM	0.0641 mL	0.3204 mL	0.6407 mL	1.2814 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.