Nemorubicin | CAS 108852-90-0

Nemorubicin

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
100mg	$1350	Check With Us
250mg	$2250	Check With Us
500mg	$3375	Check With Us

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Cat #: V4151 CAS #: 108852-90-0 Purity ≥ 98%

Description: Nemorubicin (also known as PNU152243A; Methoxymorpholinyldoxorubicin; PNU 152243; PNU-152243A), an analog of doxorubicin, is a DNA-intercalator, topoisomerase and RNA synthesis inhibitor that was undergoing development for cancer treatment.

References: Lu H, et al. Potentiation of methoxymorpholinyl doxorubicin antitumor activity by P450 3A4 gene transfer. Cancer Gene Ther. 2009 May;16(5):393-404.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	643.64
Molecular Formula	C32H37NO13
CAS No.	108852-90-0
Storage	-20℃ for 3 years in powder formrr
Storage	-80℃ for 2 years in solvent

Protocol	In Vitro	Nemorubicin is cytotoxic to 9L/3A4 cells, with an IC50 of 0.2 nM, 120-fold lower than that of P450-deficient 9L cells (IC50, 23.9 nM). Nemorubicin also potently inhibits Adeno-3A4 infected U251 cells with IC50 of 1.4 nM. P450 reductase overexpression enhances cytotoxicity of Nemorubicin
Protocol	In Vivo	Nemorubicin (60 µg/kg) induces sifnificant tumor growth delay in scid mice bearing 9L/3A4 tumors, but shows no obvious effect on the tumor growth delay of 9L tumors in mice by i.v. or intratumoral injection (i.t.). Nemorubicin (40 µg/kg, i.p.) exhibits no antitumor activity and no host toxicity in mice bearing 9L/3A4 tumors

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	1.5537 mL	7.7683 mL	15.5366 mL	31.0733 mL
5mM	0.3107 mL	1.5537 mL	3.1073 mL	6.2147 mL
10mM	0.1554 mL	0.7768 mL	1.5537 mL	3.1073 mL
20mM	0.0777 mL	0.3884 mL	0.7768 mL	1.5537 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.