TAME | CAS 901-47-3 | InvivoChem

TAME

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
500mg	$250	Check With Us
1g	$450	Check With Us
5g	$1215	Check With Us

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Cat #: V1321 CAS #: 901-47-3 Purity ≥ 98%

Description: TAME (Tosyl-L-Arginine Methyl Ester) is a potent small molecule inhibitor of APC (anaphase-promoting complex/cyclosome), which is an ubiquitin ligase with multiple subunits and can target proteins for degradation, leading to the separation of chromatids.

References: Zeng X, et al. An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. Nat Chem Biol. 2012 Feb 26;8(4):383-92.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	342.41
Molecular Formula	C14H22N4O4S
CAS No.	901-47-3
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 69 mg/mL (201.5 mM)r
	Water: 69 mg/mL (201.5 mM)r
	Ethanol: 3 mg/mL (8.76 mM)
Synonyms	Tosyl-L-Arginine Methyl Ester

Protocol	In Vitro	The absence of APC substrates, TAME ejects Cdc20 from the APC by promoting Cdc20 auto-ubiquitination in its N-terminal region. Cyclin B1 antagonizes TAME's effect by promoting binding of free Cdc20 to the APC and suppressing Cdc20 auto-ubiquitination

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.9205 mL	14.6024 mL	29.2048 mL	58.4095 mL
5mM	0.5841 mL	2.9205 mL	5.8410 mL	11.6819 mL
10mM	0.2920 mL	1.4602 mL	2.9205 mL	5.8410 mL
20mM	0.1460 mL	0.7301 mL	1.4602 mL	2.9205 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.