R547 (Ro 4584820)
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $1950 | Check With Us |
| 500mg | $2925 | Check With Us |
| 1g | $4385 | Check With Us |
: Cat #: V1559 CAS #: 741713-40-6 Purity ≥ 98%
Description: R547 (R-547; Ro4584820; R 547; Ro-4584820) is a potent, selective and ATP-competitive inhibitor of CDK1/2/4 with potential antitumor activity.
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- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 441.45 |
|---|---|
| Molecular Formula | C18H21F2N5O4S |
| CAS No. | 741713-40-6 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 60 mg/mL (135.9 mM)r |
| Water: <1 mg/mLr | |
| Ethanol: <1 mg/mL | |
| Solubility In Vivo | 1% hydroxyethyl cellulose+0.2% Tween 80: 30 mg/mL |
| Synonyms | R547; Ro4584820; R 547; Ro-4584820; R-547; Ro 4584820 |
| Protocol | In Vitro | R547 potently inhibits CDK1/cyclin B, CDK2/cyclin E, and CDK4/cyclin D1 (Ki=1-3 nM) and is inactive (Ki> 120,5 nM) of > unrelated kinases in cells. |
|---|---|---|
| In Vivo | R547 has significant in vivo efficacy and is administered orally daily and intravenously once weekly. R547 Inhibits phosphorylation of retinoblastoma proteins in tumors. | |
| Animal model | 13-14 weeks old female immunodeficient nude mice (23-25 g), with HCT116/H460a/MDA-MB-435/DU145/LOX/A549 cells xenograft. | |
| Dosages | 40 mg/kg | |
| Administration | Oral administration; daily; for 3-weeks |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.2653 mL | 11.3263 mL | 22.6526 mL | 45.3052 mL |
| 5mM | 0.4531 mL | 2.2653 mL | 4.5305 mL | 9.0610 mL |
| 10mM | 0.2265 mL | 1.1326 mL | 2.2653 mL | 4.5305 mL |
| 20mM | 0.1133 mL | 0.5663 mL | 1.1326 mL | 2.2653 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.