ML224
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 100mg | $920 | Check With Us |
| 250mg | $1600 | Check With Us |
| 500mg | $2400 | Check With Us |
: Cat #: V2924 CAS #: 1338824-21-7 Purity ≥ 98%
Description: ML224, also known as NCGC00242364 and ANTAG3, is a selective and inverse agonist of Thyroid Stimulating Hormone Receptor(TSHR).
Top Publications Citing Invivochem Products
Publications Citing InvivoChem Products
Product Promise
- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 525.59 |
|---|---|
| Molecular Formula | C31H31N3O5 |
| CAS No. | 1338824-21-7 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 10 mM in DMSOr |
| Water: <1 mg/mLr | |
| Ethanol: N/A | |
| SMILES Code | CC(NC1=CC(C)=C(OCC2=CC(C(N3CC4=CC=CO4)NC5=C(C=CC=C5)C3=O)=CC=C2OC)C(C)=C1)=O |
| Synonyms | NCGC00242364; ANTAG3; NCGC 00242364; ANTAG 3; NCGC-00242364; ANTAG-3; ML224; ML-224; ML 224 |
| Protocol | In Vitro | ML224 (0.001-100 µM; 20 min) exhibits half-maximal inhibitory doses of 2.1 µM for TSHR and greater than 30 µM for LH and FSH receptors in human embryonic kidney 293 cells |
|---|---|---|
| In Vivo | ANTAG3 (2 mg/mice; i.p. via osmotic pump; single daily for 3 days) lowers serum FT4 levels and thyroidal mRNAs for TPO and NIS in mice continuously stimulated by TRH | |
| Animal model | Female BALB/c mice (8 to 13-week-old; ~18.7 g) | |
| Dosages | 2 mg/mice | |
| Administration | Intraperitoneal injection via osmotic pump; single daily for 3 days |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 1.9026 mL | 9.5131 mL | 19.0262 mL | 38.0525 mL |
| 5mM | 0.3805 mL | 1.9026 mL | 3.8052 mL | 7.6105 mL |
| 10mM | 0.1903 mL | 0.9513 mL | 1.9026 mL | 3.8052 mL |
| 20mM | 0.0951 mL | 0.4757 mL | 0.9513 mL | 1.9026 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.