TGR5 Receptor Agonist

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
100mg	$700	Check With Us
250mg	$1350	Check With Us
500mg	$2025	Check With Us

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Cat #: V2862 CAS #: 1197300-24-5 Purity ≥ 98%

Description: TGR5 Receptor Agonist is a novel, potent and synthetic small molecule agonist of TGR5(G-protein coupled receptor19, GPCR19) agonist, it showed improved potency in the U2-OS cell assay with pEC50 of 6.8 and in melanophore cells with pEC50 of 7.5.

References: Caldwell A, Grundy L, Harrington AM, Garcia-Caraballo S, Castro J, Bunnett NW, Brierley SM. TGR5 agonists induce peripheral and central hypersensitivity to bladder distension. Sci Rep. 2022 Jun 15;12(1):9920.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	361.22
Molecular Formula	C18H14Cl2N2O2
CAS No.	1197300-24-5
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: ≥ 48 mg/mLr
	Water: <1 mg/mLr
	Ethanol: <1 mg/mL
Solubility In Vivo	O=C(C1=C(C)ON=C1C2=CC=CC=C2Cl)N(C3=CC=C(Cl)C=C3)C
Synonyms	TGR5 Receptor Agonist

Protocol	In Vivo	TGR5 Receptor Agonist (CCDC) activates directly a sub-population of bladder-innervating dorsal root ganglia (DRG) neurons and a small percentage of non-neuronal cells in Trpv1-/- mice
	Animal model	Female C57BL/6J mice [12-18 weeks; TRPV1 knockout (trpv1-/-), TRPA1 knockout (trpa1-/-), or TGR5 knockout (Gpbar1-/-)]
	Dosages	100 µM, 100 µL
	Administration	Infused gently, to fill but not fully distend the bladder, and allowed to incubate for 5 min

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.7684 mL	13.8420 mL	27.6840 mL	55.3679 mL
5mM	0.5537 mL	2.7684 mL	5.5368 mL	11.0736 mL
10mM	0.2768 mL	1.3842 mL	2.7684 mL	5.5368 mL
20mM	0.1384 mL	0.6921 mL	1.3842 mL	2.7684 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.