Anisodamine
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
Size | Price | Stock |
---|---|---|
10g | $450 | Check With Us |
25g | $800 | Check With Us |
50g | $1200 | Check With Us |
Cat #: V10759 CAS #: 55869-99-3 Purity ≥ 99%
Description: Anisodamine is a ntural product extracted from Chinese solanacea plant, acting as a weak inhibitor/antagonist of alpha-1 adrenergic receptors and mAChRs.
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- Related Biological Data
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Molecular Weight (MW) | 305.37 |
---|---|
Molecular Formula | C17H23NO4 |
CAS No. | 55869-99-3 |
Protocol | In Vitro | Anisodamine (100 μg/mL; 20 minutes; RAW264.7 cells) pretreatment for 20 minutes before Ach results in significantly attenuates average fluorescence intensity of α-bungarotoxin binding compared with Ach alone |
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In Vivo | Anisodamine (50 mg/kg; i.p.; 72 hours) markedly decreases the mortality to 20% | |
Animal model | LPS-Induced Shock Mice | |
Dosages | LPS-Induced Shock Mice | |
Administration | tion: I.p. |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
Solvent volume to be added | Mass (the weight of a compound) | |||
---|---|---|---|---|
Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
1mM | 3.2747 mL | 16.3736 mL | 32.7472 mL | 65.4943 mL |
5mM | 0.6549 mL | 3.2747 mL | 6.5494 mL | 13.0989 mL |
10mM | 0.3275 mL | 1.6374 mL | 3.2747 mL | 6.5494 mL |
20mM | 0.1637 mL | 0.8187 mL | 1.6374 mL | 3.2747 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.