Melatonin | CAS 73-31-4

Melatonin

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
10g	$200	Check With Us
25g	$370	Check With Us
50g	$555	Check With Us

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Cat #: V1311 CAS #: 73-31-4 Purity ≥ 98%

Description: Melatonin (Circadin; 5-Methoxy-N-acetyltryptamine; NSC-56423; NSC 113928; Regulin), an endogenous indoleamine neurohormone associated with control of the sleep–wake cycle, is a MT receptor agonist used as a dietary supplement for controlling of circadian rhythm.

References: Kilic U, et al. Particular phosphorylation of PI3K/Akt on Thr308 via PDK-1 and PTEN mediates melatonin's neuroprotective activity after focal cerebral ischemia in mice. Redox Biol. 2017 Apr 5;12:657-665

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	232.28
Molecular Formula	C13H16N2O2
CAS No.	73-31-4
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 47 mg/mL (202.3 mM)r
	Water: <1 mg/mLr
	Ethanol: 47 mg/mL (202.3 mM)
Synonyms	Melatonin; Melatonine; N-Acetyl-5-methoxytryptamine; Circadin; 5-Methoxy-N-acetyltryptamine; NSC 56423; NSC 113928; Regulin

Protocol	In Vivo	Melatonin increases the levels of activated PTEN, RSK-1, mTOR and AMPKα kinases, mildly inhibits ERK-1/2 phosphorylation and Bad phosphorylation, significantly inhibits phosphorylations of S6 Ribosomal Protein, 4E-BP1, GSK-3α and GSK-3β, and slightly increases PRAS40 phosphorylation in animals

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	4.3051 mL	21.5257 mL	43.0515 mL	86.1030 mL
5mM	0.8610 mL	4.3051 mL	8.6103 mL	17.2206 mL
10mM	0.4305 mL	2.1526 mL	4.3051 mL	8.6103 mL
20mM	0.2153 mL	1.0763 mL	2.1526 mL	4.3051 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.