PF-5274857
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $1350 | Check With Us |
| 500mg | $1920 | Check With Us |
| 1g | $2880 | Check With Us |
: Cat #: V1336 CAS #: 1373615-35-0 Purity ≥ 98%
Description: PF-5274857 (PF5274857; PF 5274857) is a novel, potent, orally bioavailable and selective Smoothened (Smo) antagonist with potential antineoplastic activity.
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- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 436.96 |
|---|---|
| Molecular Formula | C20H25ClN4O3S |
| CAS No. | 1373615-35-0 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: 93 mg/mL (212.8 mM)r |
| Water:<1 mg/mLr | |
| Ethanol: 93 mg/mL (212.8 mM) | |
| Solubility In Vivo | Saline: 30 mg/mL |
| Synonyms | PF-5274857; PF 5274857; PF5274857 |
| Protocol | In Vitro | PF-5274857 completely inhibits Shh-induced Hh pathway activity with an IC50 of 2.7±1.4 nM measured by the transcriptional activity of Smo downstream gene Gli1 in MEF cells |
|---|---|---|
| In Vivo | PF-5274857 (1-30 mg/kg; p.o. once daily for 6 days) shows robust antitumor efficacy and correlation between PK and PD in medulloblastoma allograft mice models | |
| Animal model | Severe combined immunodeficient (SCID)-beige mice (6-8 weeks old) are genetically engineered | |
| Dosages | 0, 1, 5, 10, 30 mg/kg | |
| Administration | P.o. once daily for 6 days |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.2885 mL | 11.4427 mL | 22.8854 mL | 45.7708 mL |
| 5mM | 0.4577 mL | 2.2885 mL | 4.5771 mL | 9.1542 mL |
| 10mM | 0.2289 mL | 1.1443 mL | 2.2885 mL | 4.5771 mL |
| 20mM | 0.1144 mL | 0.5721 mL | 1.1443 mL | 2.2885 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.