Clemizole | CAS 442-52-4 (free base); 1163-36-6 (HCl salt)

Clemizole

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
250mg	$750	Check With Us
500mg	$1250	Check With Us
1g	$1875	Check With Us

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Cat #: V3412 CAS #: 442-52-4 (free base); 1163-36-6 (HCl salt) Purity ≥ 98%

Description: Clemizole (also known as AL 20, P 48, and/or Reactrol), is a potent inhibitor of transient receptor potential channel TRPC5 (Canonical transient receptor potential channel 5) and also an H1 histamine receptor antagonist.

References: Nishimura T, et al. Using chimeric mice with humanized livers to predict human drug metabolism and a drug-drug interaction. J Pharmacol Exp Ther. 2013 Feb;344(2):388-96.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	325.84
Molecular Formula	C₁₉H₂₀ClN₃
CAS No.	442-52-4 (free base); 1163-36-6 (HCl salt)
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 10 mMr
	Water: <1 mg/mLr
	Ethanol:
SMILES Code	ClC1=CC=C(C=C1)CN2C3=CC=CC=C3N=C2CN4CCCC4
Synonyms	Clemizole; AL 20; AL20; AL-20; P 48; Reactrol

Protocol	In Vivo	Clemizole hydrochloride has an unexpectedly short plasma half-life (measured at 0.15 hours); it is very rapidly biotransformed into a glucuronide (M14) and a dealkylated metabolite (M12) and into a variety of lesser metabolites in C57BL/6J mice

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.0690 mL	15.3450 mL	30.6899 mL	61.3798 mL
5mM	0.6138 mL	3.0690 mL	6.1380 mL	12.2760 mL
10mM	0.3069 mL	1.5345 mL	3.0690 mL	6.1380 mL
20mM	0.1534 mL	0.7672 mL	1.5345 mL	3.0690 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.