Nalidixic acid (NSC-82174)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
50g	$330	Check With Us
100g	$450	Check With Us
200g	$675	Check With Us

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Cat #: V1407 CAS #: 389-08-2 Purity ≥ 98%

Description: Nalidixic acid (formerly known as NSC-82174; NSC82174; Nevigramon, Neggram, Wintomylon, WIN 18,320), a synthetic 1,8-naphthyridine antimicrobial agent used to treat infections, is the first synthetic quinolone-based antibiotic with a narrow spectrum of bacteriocidal effects.

References: antibiotic, DNA gyrase, susceptible bacteria, Escherichia coli, Klebsiella pneuiiioniae, Aerobacter aeroyenes, Proteus spp, Salmonella spp.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	232.24
Molecular Formula	C12H12N2O3
CAS No.	389-08-2
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 6 mg/mL (25.8 mM)r
	Water: <1 mg/mLr
	Ethanol: <1 mg/mL
Synonyms	NSC-82174; Nalidixic Acid, Nevigramon, NSC 82174; NSC82174; Neggram, Wintomylon, WIN 18,320

Protocol	In Vitro	Nalidixic acid is against a variety of microorganisms, it is against with Escherichia coli, Pasteurella spp., Klebsiella pneuiiioniae, Aerobacter aeroyenes, Proteus spp., Salmonella spp., Shigella spp. and Brucella spp. with MIC values of 5.0-12.5 μg/ml, 0.5-2.5 μg/ml, 0.8-25.0 μg/ml, 1.0-25.0 μg/ml, 1.25-30.0 μg/ml, 8-3.2 μg/ml, and 7.5-10.0 μg/ml, respectively
Protocol	In Vivo	The in vivo activity of Nalidixic acid is most pronounced against Gram-negative bacteria, while Gram-positive organisms are generally more resistant. Maximal activity is observed against systemic infections caused by E. coli, A. aerobacter, Proteus mirabilis, Shigella fkxneri, the ED50 values are 25 mg/kg, 60 mg/kg, 50 mg/kg, and 62 mg/kg, respectively

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	4.3059 mL	21.5295 mL	43.0589 mL	86.1178 mL
5mM	0.8612 mL	4.3059 mL	8.6118 mL	17.2236 mL
10mM	0.4306 mL	2.1529 mL	4.3059 mL	8.6118 mL
20mM	0.2153 mL	1.0765 mL	2.1529 mL	4.3059 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.