Amonafide (AS1413)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
250mg	$950	Check With Us
500mg	$1450	Check With Us
1g	$2175	Check With Us

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Cat #: V1417 CAS #: 69408-81-7 Purity ≥ 98%

Description: Amonafide (formerly NSC308847, AS1413; AS-1413; NSC-308847; Amonafide; Nafidimide; Benzisoquinolinedione; Quinamed; Xanafide), an imide derivative of naphthalic acid, is an inhibitor of topoisomerase II (Topo II) and also a DNA intercalator being investigated for the treatment of cancer.

References: Allen SL, et al. Amonafide: a potential role in treating acute myeloid leukemia. Expert Opin Investig Drugs. 2011 Jul;20(7):995-1003.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	283.33
Molecular Formula	C16H17N3O2
CAS No.	69408-81-7
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 57 mg/mL (201.2 mM)r
	Water: <1 mg/mLr
	Ethanol: 4 mg/mL (14.1 mM)
Synonyms	AS1413; NSC308847, AS1413; NSC-308847, AS-1413; NSC 308847, AS 1413; Amonafide; Nafidimide; Benzisoquinolinedione; Quinamed; Xanafide

Protocol	In Vitro	Amonafide is a topoisomerase II inhibitor and DNA intercalator that induces apoptotic signaling by blocking the binding of Topo II to DNA

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	3.5295 mL	17.6473 mL	35.2945 mL	70.5891 mL
5mM	0.7059 mL	3.5295 mL	7.0589 mL	14.1178 mL
10mM	0.3529 mL	1.7647 mL	3.5295 mL	7.0589 mL
20mM	0.1765 mL	0.8824 mL	1.7647 mL	3.5295 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.