WAY-316606
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 100mg | $850 | Check With Us |
| 250mg | $1550 | Check With Us |
| 500mg | $2325 | Check With Us |
: Cat #: V4123 CAS #: 915759-45-4 Purity ≥ 98%
Description: WAY 316606 is a novel and potent small molecule inhibitor of the secreted protein sFRP-1 (secreted frizzled-related protein) which is an endogenous antagonist of the secreted glycoprotein Wnt.
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| Molecular Weight (MW) | 448.48 |
|---|---|
| Molecular Formula | C18H19F3N2O4S2 |
| CAS No. | 915759-45-4 |
| Storage | -20℃ for 3 years in powder formr |
| -80℃ for 2 years in solvent | |
| Solubility In Vitro | DMSO: ≥ 85 mg/mLr |
| Water: < 1 mg/mLr | |
| Ethanol: N/A | |
| SMILES Code | O=S(C1=CC(S(=O)(C2=CC=CC=C2)=O)=CC=C1C(F)(F)F)(NC3CCNCC3)=O |
| Synonyms | WAY316606; WAY-316606; WAY 316606 |
| Protocol | In Vitro | The EC50 of WAY-316606 for Wnt-Luciferase Activity from U2-OS Cells is 0.65 μM. WAY-316606 binds to secreted frizzled-related protein (sFRP)-1 inhibitor with a KD of 0.08 μM and inhibits sFRP-1 with an EC50 of 0.65 μM. WAY-316606 also binds to sFRP-2, albeit over 10 times weaker with a KD of 1 μM. Using a fluorescence polarization binding assay that employs a fluorescent probe compound and purified human sFRP-1 protein in a competitive-binding format, the IC50 for WAY-316606 is 0.5 μM |
|---|---|---|
| In Vivo | WAY-316606 increases bone formation when tested in a neonatal murine calvarial assay. WAY-316606 increases total bone area up to 60% in a dose-dependent manner with an EC50 of about 1 nM. WAY-316606 has good aqueous solubility, moderate to low inhibition of cytochrome p450 isozymes (3A4, 2D6, 2C9) and good stability in rat and human liver microsomes (t1/2>60 min in each species). In female Sprague-Dawley rats, WAY-316606 exhibits high plasma clearance (77 mL/min/kg, greater than hepatic blood flow) following a single intravenous bolus dose (2 mg/kg), which results in a rapid decline of drug exposure in the plasma despite the route of administration |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.2298 mL | 11.1488 mL | 22.2975 mL | 44.5951 mL |
| 5mM | 0.4460 mL | 2.2298 mL | 4.4595 mL | 8.9190 mL |
| 10mM | 0.2230 mL | 1.1149 mL | 2.2298 mL | 4.4595 mL |
| 20mM | 0.1115 mL | 0.5574 mL | 1.1149 mL | 2.2298 mL |
The molarity calculator equation
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The dilution calculator equation
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Step One: Enter information below
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Step Two: Enter the in vivo formulation
%DMSO
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%Tween 80
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%ddH2O
Calculation Results:
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Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
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mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.