AF-353 (Ro-4)
This product is for research use only, not for human use. We do not sell to patients.
For small sizes, please check our retail website as below: www.invivochem.com
| Size | Price | Stock |
|---|---|---|
| 250mg | $980 | To Be Confirmed |
| 500mg | $1550 | To Be Confirmed |
| 1g | $2325 | To Be Confirmed |
: Cat #: V5171 CAS #: 865305-30-2 Purity ≥ 98%
Description: AF-353 (Ro-4; AF353) is a novel, highly potent, selective and orally bioavailable P2X3/P2X2/3 receptor antagonist with a pIC50 of 8.0 for both human and rat P2X3, and with a pIC50 of 7.3 for human P2X2/3.
Top Publications Citing Invivochem Products
Publications Citing InvivoChem Products
Product Promise
- Physicochemical and Storage Information
- Protocol
- Related Biological Data
- Stock Solution Preparation
- Quality Control Documentation
| Molecular Weight (MW) | 400.2205 |
|---|---|
| Molecular Formula | C14H17IN4O2 |
| CAS No. | 865305-30-2 |
| Storage | -20℃ for 3 years in powder formrr |
| -80℃ for 2 years in solvent | |
| SMILES Code | NC1=NC=C(OC2=CC(I)=C(OC)C=C2C(C)C)C(N)=N1 |
| Synonyms | Ro-4; Ro 4; Ro4; AF353; AF-353; AF 353 |
| Protocol | In Vivo | AF-353 (Ro-4) does not compromise oxygen levels or cardiac functio |
|---|---|---|
| Animal model | Female Sprague-Dawley rats (250–300 g) bearing SCI | |
| Dosages | 10 mg/kg, 20 mg/kg | |
| Administration | Intravenous injection; interval of 90 minutes, for 4 hours to 6 hours |
These protocols are for reference only. InvivoChem does not
independently validate these methods.
| Solvent volume to be added | Mass (the weight of a compound) | |||
|---|---|---|---|---|
| Mother liquor concentration | 1mg | 5mg | 10mg | 20mg |
| 1mM | 2.4986 mL | 12.4931 mL | 24.9862 mL | 49.9725 mL |
| 5mM | 0.4997 mL | 2.4986 mL | 4.9972 mL | 9.9945 mL |
| 10mM | 0.2499 mL | 1.2493 mL | 2.4986 mL | 4.9972 mL |
| 20mM | 0.1249 mL | 0.6247 mL | 1.2493 mL | 2.4986 mL |
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start)
×
Volume(start)
=
Concentration(final)
×
Volume(final)
This equation is commonly abbreviated as: C1 V1 = C2 V2
Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg
Average weight of animals g
Dosing volume per animal µL
Number of animals
Step Two: Enter the in vivo formulation
%DMSO
+
%
+
%Tween 80
+
%ddH2O
Calculation Results:
Working concentration:
mg/ml;
Method for preparing DMSO master liquid:
mg
drug pre-dissolved in
µL
DMSO(Master liquid concentration
mg/mL)
,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:
Take
µL
DMSO master liquid, next add
µL
PEG300, mix and clarify, next add
µL
Tween 80,mix and clarify, next add
µL
ddH2O,mix and clarify.
Note:
- (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
- (2) Be sure to add the solvent(s) in order.