Arecoline HBr

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
25g	$450	Check With Us
50g	$750	Check With Us
100g	$1125	Check With Us

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Cat #: V1187 CAS #: 300-08-3 Purity ≥ 98%

Description: Arecoline HBr (NSC56321, NSC-56321), the hydrobromide salt of Arecoline which is a naturally occurring alkaloid found in the betel nut of the Areca palm, is a potent agonist of muscarinic acetylcholine receptor with the potential to be used in treating dementia.

References: Thangjam GS, et, al. Regulation of oxidative-stress responsive genes by arecoline in human keratinocytes. J Periodontal Res. 2009 Oct;44(5):673-82.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	236.11
Molecular Formula	C8H13NO2.HBr
CAS No.	300-08-3
Storage	-20℃ for 3 years in powder formrr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 47 mg/mL (199.1 mM)rr
	Water: 47 mg/mL (199.1 mM)rr
	Ethanol: 10 mg/mL (42.4 mM)
SMILES Code	O=C(C1=CCCN(C)C1)OC.Br
Synonyms	NSC 56321, Arecoline Hydrobromide, Arecoline HBr, NSC56321, NSC-56321, Arecoline

Protocol	In Vitro	Arecoline hydrobromide induces the generation of reactive oxygen species and cell cycle arrest at the G1/G0 phase in HaCaT cells without affecting the expression of p21/Cip1. Arecoline hydrobromide-induced epithelial cell death at higher concentrations is caused by oxidative trauma without eliciting apoptosis. Arecoline hydrobromide upregulates the expression of the following stress-responsive genes: hemeoxygenase-1; ferritin light chain; glucose-6-phosphate dehydrogenase; glutamatecysteine ligase catalytic subunit; and glutathione reductase

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	4.2353 mL	21.1766 mL	42.3531 mL	84.7063 mL
5mM	0.8471 mL	4.2353 mL	8.4706 mL	16.9413 mL
10mM	0.4235 mL	2.1177 mL	4.2353 mL	8.4706 mL
20mM	0.2118 mL	1.0588 mL	2.1177 mL	4.2353 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.