Evans Blue

This product is for research use only, not for human use. We do not sell to patients.

Evans Blue
For small sizes, please check our retail website as below: www.invivochem.com
Size Price Stock
25g$380To Be Confirmed
50g$650To Be Confirmed
100g$975To Be Confirmed

Cat #: V2710 CAS #: 314-13-6 Purity ≥ 98%

Description: Evans Blue (Direct Blue 53) is a potent inhibitor of the uptake of L-glutamate into synaptic vesicles, it is also an AMPA/kainate receptor antagonist.

References: Bioorg Med Chem Lett. 2004 Dec 20;14(24):6123-7; J Pharmacol Exp Ther. 2005 Nov;315(2):965-9.

Top Publications Citing Invivochem Products
Publications Citing InvivoChem Products

Product Promise

Promise
Molecular Weight (MW)960.81
Molecular FormulaC34H24N6O14S4.4Na
CAS No.314-13-6
Storage-20℃ for 3 years in powder formrr
-80℃ for 2 years in solvent
Solubility In VitroDMSO: 100 mg/mL (104.1 mM)rr
Water: 100 mg/mL (104.1 mM)rr
Ethanol: <1 mg/mL
SMILES Code[O-]S(C1=CC(S([O-])(=O)=O)=C2C=CC(/N=N/C3=CC=C(C4=CC=C(/N=N/C5=C(O)C6=C(N)C(S([O-])(=O)=O)=CC(S([O-])(=O)=O)=C6C=C5)C(C)=C4)C=C3C)=C(O)C2=C1N)(=O)=O.[Na+].[Na+].[Na+].[Na+]
SynonymsDirect Blue 53
Preparing Stock Solutions
Solvent volume to be added Mass (the weight of a compound)
Mother liquor concentration 1mg5mg10mg20mg
1mM1.0408 mL5.2039 mL10.4079 mL20.8158 mL
5mM0.2082 mL1.0408 mL2.0816 mL4.1632 mL
10mM0.1041 mL0.5204 mL1.0408 mL2.0816 mL
20mM0.0520 mL0.2602 mL0.5204 mL1.0408 mL
Quality Control Documentation
The molarity calculator equation
Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)
Mass
=
Concentration
×
Volume
×
Molecular Weight*
The dilution calculator equation
Concentration(start) × Volume(start) = Concentration(final) × Volume(final)

This equation is commonly abbreviated as: C1 V1 = C2 V2

Concentration(start)
C1
×
Volume(start)
V1
=
Concentration(final)
C2
×
Volume(final)
V2
Step One: Enter information below
Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals
Step Two: Enter the in vivo formulation
%DMSO + % + %Tween 80 + %ddH2O

Calculation Results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH2O,mix and clarify.
Note:
  • (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
  • (2) Be sure to add the solvent(s) in order.