Sulfosuccinimidyl Oleate sodium

This product is for research use only, not for human use. We do not sell to patients.

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Size	Price	Stock
100mg	$1150	Check With Us
200mg	$1725	Check With Us
500mg	$2910	Check With Us

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Cat #: V60029 CAS #: 1212012-37-7 Purity ≥ 99%

Description: Sulfosuccinimidyl oleate sodium (SSO sodium salt) is a long chain fatty acid acting as an irreversible inhibitor of the fatty acid translocase CD36, blocking uptake of oleate, linoleate, or stearate by about 65% when added at 200 µM to adipocytes.

References: Dhungana H, et al. Sulfosuccinimidyl oleate sodium is neuroprotective and alleviates stroke-induced neuroinflammation. J Neuroinflammation. 2017 Dec 4;14(1):237.

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Protocol
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Stock Solution Preparation
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Molecular Weight (MW)	481.58
Molecular Formula	C22H36NNaO7S
CAS No.	1212012-37-7
SMILES Code	CCCCCCCC/C=C\CCCCCCCC(ON1C(C(S(O)(=O)=O)CC1=O)=O)=O.[Na+]
Synonyms	Sulfosuccinimidyl oleate sodium; Sulfo-N-succinimidyl oleate sodium

Protocol	In Vitro	Sulfosuccinimidyl oleate (50 μM, 24 hours) co-treatment significantly reduces the LPS+IFNγ-induced expression of NOS2 and COX-2 in BV2 cells. Western blot analysis reveals a significant LPS/IFNγ-induced upregulation in the phosphorylated form of the p38, which is prevented by co-treatment with Sulfosuccinimidyl oleate (50 μM, 24 hours). Sulfosuccinimidyl oleate (20 μM and 50 μM, 24 hours) alone does not alter the cellular viability. Exposure to 100 ng/ml LPS+5 ng/mL IFNγ modestly, yet significantly reduces the viability of the BV2 cells. Co-treatment with Sulfosuccinimidyl oleate prevents the LPS+IFNγ-induced reduction in the cell viability.
Protocol	In Vivo	Sulfosuccinimidyl oleate (50 mg/kg; administered once by single oral gavage) significantly reduces the cortical ischemic infarct size compared to vehicle-treated controls in male BALB/cABom mice with pMCAo model. In addition, Sulfosuccinimidyl oleate at 50 mg/kg is suitable to see a beneficial effect after stroke.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.0765 mL	10.3825 mL	20.7650 mL	41.5300 mL
5mM	0.4153 mL	2.0765 mL	4.1530 mL	8.3060 mL
10mM	0.2076 mL	1.0382 mL	2.0765 mL	4.1530 mL
20mM	0.1038 mL	0.5191 mL	1.0382 mL	2.0765 mL

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.