20-Hydroxyecdysone (Crustecdysone)

This product is for research use only, not for human use. We do not sell to patients.

For small sizes, please check our retail website as below: www.invivochem.com

Size	Price	Stock
250mg	$625	Check With Us
500mg	$1025	Check With Us
1g	$1535	Check With Us

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Cat #: V2376 CAS #: 5289-74-7 Purity ≥ 98%

Description: 20-Hydroxyecdysone (also known as B-ecdysone, Crustecdysone, Commisterone Ecdysterone, 20E) is a naturally occurring ecdysteroid hormone which controls the ecdysis (moulting) and metamorphosis of arthropods.

References: Liu H, et al. E93 predominantly transduces 20-hydroxyecdysone signaling to induce autophagy and caspase activity in Drosophila fat body. Insect Biochem Mol Biol. 2014 Feb;45:30-9.

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Physicochemical and Storage Information
Protocol
Related Biological Data
Stock Solution Preparation
Quality Control Documentation

Molecular Weight (MW)	480.63
Molecular Formula	C27H44O7
CAS No.	5289-74-7
Storage	-20℃ for 3 years in powder formr
Storage	-80℃ for 2 years in solvent
Solubility In Vitro	DMSO: 96 mg/mL (199.7 mM)r
	Water: <1 mg/mLr
	Ethanol: 96 mg/mL (199.7 mM)
Solubility In Vivo	1% DMSO+30% polyethylene glycol+1% Tween 80: 30 mg/mL
SMILES Code	CC(C)(O)CC[C@@H](O)[C@](C)(O)[C@H]1CC[C@@]2(O)C3=CC([C@]4([H])C[C@@H](O)[C@@H](O)C[C@]4(C)[C@@]3([H])CC[C@]12C)=O
Synonyms	B-ecdysone,Commisterone; B-ecdysone, Crustecdysone, Commisterone Ecdysterone, 20E

Preparing Stock Solutions

Solvent volume to be added	Mass (the weight of a compound)
Mother liquor concentration	1mg	5mg	10mg	20mg
1mM	2.0806 mL	10.4030 mL	20.8060 mL	41.6121 mL
5mM	0.4161 mL	2.0806 mL	4.1612 mL	8.3224 mL
10mM	0.2081 mL	1.0403 mL	2.0806 mL	4.1612 mL
20mM	0.1040 mL	0.5202 mL	1.0403 mL	2.0806 mL

Quality Control Documentation	Select Batch Purity ≥ 98% COA MSDS NMR HPLC

The molarity calculator equation

Mass(g) = Concentration(mol/L) × Volume(L) × Molecular Weight(g/mol)

Mass

Concentration

Volume

Molecular Weight*

The dilution calculator equation

Concentration_(start) × Volume_(start) = Concentration_(final) × Volume_(final)

This equation is commonly abbreviated as: C₁ V₁ = C₂ V₂

Concentration_(start)

Volume_(start)

Concentration_(final)

Volume_(final)

Step One: Enter information below

Dosage mg/kg Average weight of animals g Dosing volume per animal µL Number of animals

Step Two: Enter the in vivo formulation

%DMSO + % + %Tween 80 + %ddH₂O

Calculation Results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in µL DMSO(Master liquid concentration mg/mL) ,Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation: Take µL DMSO master liquid, next add µL PEG300, mix and clarify, next add µL Tween 80,mix and clarify, next add µL ddH₂O,mix and clarify.

Note:

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.